Fuel ethanol production using novel carbon sources and fermentation medium optimization with response surface methodology
Keywords:
gluconate salts, ethanol, response surface methodology, medium optimization, biofuelAbstract
In this study, ethanol production abilities of the novel carbon sources: sodium and calcium gluconate in different minimal and rich media were compared with glucose using Escherichia coli KO11. The strain produced higher ethanol yield in the rich medium Luria-Bertani (LB) than the other two minimal media: corn steep liquor (CSL) and M9 for two substrates (sodium and calcium gluconate). Additionally, higher ethanol yields were achieved when the strain was grown in LB and M9 medium with calcium gluconate than sodium gluconate, while the ethanol yields were similar when both sodium and calcium gluconate were added into CSL medium respectively. Response surface methodology was used to optimize the fermentation medium components for enhancing ethanol production using strain E. coli KO11 in CSL medium with calcium gluconate as the substrate in batch culture. The concentration of the potassium phosphate buffer is the only significant factor among five factors considered. A quadratic model was developed to describe the relationship between ethanol production and the factors. The optimal conditions predicted for five factors were 14.38 g/L CSL, 0.0398 g/L FeCl3•6H2O, 1.12 g/L MgSO4•6H2O, 15.41 g/L (NH4)2SO4, and 1.58/1.26 g/L KH2PO4/K2HPO4 (2:1 molar ratio). The highest ethanol concentration under optimal conditions was 31.5 g/L, which was 5.6 g/L higher than that from the same fermentation concentration of calcium gluconate in LB media. The high correlation between the predicted and experimental values confirmed the validity of the model.References
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[3] Lynd L R, van Zyl W H, McBride J E, Laser M. Consolidated bioprocessing of cellulosic biomass: An update. Current Opinion in Biotechnology, 2005; 16(5): 577-583.
[4] Lynd L R, Wyman C E, Gerngross T U. Biocommodity engineering. Biotechnology Progress, 1999; 15(5): 777-793.
[5] Fan Z, Wu W, Hildebrand A, Kasuga T, Zhang R, Xiong X. A novel biochemical route for fuels and chemicals production from cellulosic biomass. PLOS One, 2012; 7(2): e31693.
[6] Ohta K, Beall D S, Mejia J P, Shanmugam K T, Ingram L O. Genetic improvement of Escherichia coli for ethanol production: chromosomal integration of Zymomonas mobilis genes encoding pyruvate decarboxylase and alcohol dehydrogenase II. Applied Environmental Microbiology, 1991; 57(4): 893-900.
[7] Underwood S A, Buszko M L, Shanmugam K T, Ingram L O. Flux through citrate synthase limits the growth of ethanologenic Escherichia coli KO11 during xylose fermentation. Applied Environmental Microbiology, 2002; 68(3): 1071-1081.
[8] Okuda N, Ninomiya K, Katakura Y, Shioya S. Strategies for reducing supplemental medium cost in bioethanol production from waste house wood hydrolysate by ethanologenic Escherichia coli: Inoculum size increase and coculture with Saccharomyces cerevisiae. Journal of Bioscience and Bioengineering, 2008; 105(2): 90-96.
[9] York S W, Ingram L O. Soy-based medium for ethanol production by Escherichia coli KO11. Journal of Industrial Microbiology, 1996; 16(6): 374-376.
[10] York S W, Ingram L O. Ethanol production by recombinant Escherichia coli KO11 using crude yeast autolysate as a nutrient supplement. Biotechnology Letters, 1996; 18(6): 683-688.
[11] de Carvalho Lima K G, Takahashi C M, Alterthum F. Ethanol production from corn cob hydrolysates by Escherichia coli KO11. Journal of Industrial Microbiology and Biotechnology, 2002; 29(3): 124-128.
[12] Kim N J, Li H, Jung K, Chang H N, Lee P C. Ethanol production from marine algal hydrolysates using Escherichia coli KO11. Bioresource Technology, 2001; 102(16): 7466-7469.
[13] Asghari A, Bothast R J, Doran J B, Ingram L O. Ethanol production from hemicellulose hydrolysates of agricultural residues using genetically engineered Escherichia coli strain KO11. Journal of Industrial Microbiology, 1996; 16: 42-47.
[14] Grohmann K, Cameron R G, Buslig B S. Fermentation of sugars in orange peel hydrolysates to ethanol by recombinant Escherichia coli KO11. Applied Biochemisty and Biotechnology, 1995; 51(2): 423-435.
[15] Takahashi C M, de Carvalho Lima K G, Takahashi D F, Alterthum F. Fermentation of sugar cane bagasse hemicellulosic hydrolysate and sugar mixtures to ethanol by recombinant Escherichia coli KO11. World Journal of Microbiology and Biotechnology, 2000; 16(8): 829-834.
[16] Orencio-Trejo M, Flores N, Escalante A, Hernandez-Chavez G, Bolivar F, Gosset G, et al. Metabolic regulation analysis of an ethanologenic Escherichia coli strain based on RT-PCR and enzymatic activities. Biotechnology for Biofuels, 2008; 1(1): 1-8.
[17] Martinez A, York S W, Yomano L P, Pineda V L, Davis F C, Shelton J C, et al. Biosynthetic burden and plasmid burden limit expression of chromosomally integrated heterologous genes (pdc, adhB) in Escherichia coli. Biotechnology Progress, 1999; 15(5): 891-897.
[18] Eisenberg R C, Dobrogos W J. Gluconate metabolism in Escherichia coli. Journal of Bacteriology, 1967; 93(3):
941-947.
[19] Kornberg H L, Soutar A K. Utilization of gluconate by Escherichia coli - Induction of gluconate kinase and 6-phosphogluconate dehydratase activities. Biochemical Journal, 1972; 134(2): 489-498.
[20] Bachi B, Kornberg H L. Utilization of gluconate by Escherichia coli-Role of adenosine 3'-5'-cyclic monophosphate in induction of gluconate catabolism. Biochemical Journal, 1975; 150(1): 123-128.
[21] Underwood S A, Shanmugum K T, Ingram L O. Osmoprotectants stimulate growth and ethanol production by ethanologenic Escherichia coli. Abstracts of the General Meeting of the American Society for Microbiology, 2003; 103: 0-060.
[22] Martinez A, Grabar T B, Shanmugam K T, Yomano L P, York S W, Ingram L O. Low salt medium for lactate and ethanol production by recombinant Escherichia coli B. Biotechnology Letters, 2007, 29(3): 397-404.
[23] Csonka L N. Physiological and genetic responses of bacteria to osmotic stress. Microbiological Reviews, 1989; 53(1): 121-147.
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Published
2013-06-18
How to Cite
Wu, W. (2013). Fuel ethanol production using novel carbon sources and fermentation medium optimization with response surface methodology. International Journal of Agricultural and Biological Engineering, 6(2), 42–53. Retrieved from https://ijabe.migration.pkpps03.publicknowledgeproject.org/index.php/ijabe/article/view/647
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Renewable Energy and Material Systems
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