Bacillus amyloliquefaciens (IAE635) and their metabolites could purify pollutants, Vibrio spp. and coliform bacteria in coastal aquaculture wastewater
Keywords:
coastal aquaculture, Bacillus amyloliquefaciens and their metabolites (MP), bio-flocculants, chemical pollutants, Vibrio spp., coliform bacteria, wastewater purificationAbstract
High-density aquaculture often causes the emission of polluted water to the marine environment in the coastal areas of China. To solve the aquaculture-related water quality problems, it is appropriate to adapt eco-friendly methods, such as using microbes and their metabolic products to purify polluted water. In this study, the purifying effects of Bacillus amyloliquefaciens (IAE635) metabolites (poly-γ-glutamic acid; PP) and IAE635 combined with their metabolites (MP) on turbidity, COD, NO3–-N, NH4+-N, Vibrio spp. and coliform bacteria in coastal aquaculture wastewater were conducted in the lab and in situ ponds. The results showed that the removal of turbidity, COD, NH4+-N and NO3–-N with PP and MP was more significant (p < 0.05). Compared to Control treatment (Co), the turbidity, COD, NH4+-N and NO3–-N concentrations at 24th hour were evidently reduced by 86.6%, 87.5%, 83.3% and 58.0% for PP, 87.9%, 93.5%, 86.5% and 78.0% for MP, respectively. The populations of water pathogens under PP and MP were also significantly (p < 0.05) removed compared with those of Co; at 24th hour, the Vibrio spp. and coliform bacteria were decreased by 68.7% and 66.3% for PP, 75.0% and 67.1% for MP, respectively. The water purifying effect of MP was slightly better than that of PP. In situ pond purification test demonstrated that MP significantly lowered the concentrations of turbidity, COD, NH4+-N, NO3–-N and NO2–-N, which was more effective than EM. A significantly higher (p < 0.05) γ-PGA concentration and the total bacterial population for MP compared to PP indicated that MP purifies the coastal aquaculture wastewater by both flocculation and microbial decomposition. The application of MP will benefit the aquaculture industry by providing a novel method for the removal of chemical pollutants and pathogens. Keywords: coastal aquaculture, Bacillus amyloliquefaciens and their metabolites (MP), bio-flocculants, chemical pollutants, Vibrio spp., coliform bacteria, wastewater purification DOI: 10.25165/j.ijabe.20211402.6082 Citation: Shao Y L, Zhong H, Wang L K, Elbashier M M A. Bacillus amyloliquefaciens (IAE635) and their metabolites could purify pollutants, Vibrio spp. and coliform bacteria in coastal aquaculture wastewater. Int J Agric & Biol Eng, 2021; 14(2): 205–210.References
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[2] Crab R, Defoirdt T, Bossier P, Verstraete W. Biofloc technology in aquaculture: Beneficial effects and future challenges. Aquaculture, 2012; 3(5): 351–356.
[3] Li G, Bai X, Huo S, Huang Z. Fast pyrolysis of LERDADEs for renewable biofuels. IET Renewable Power Generation, 2020; 14(6): 959–967.
[4] Boopathy R, Kern C, Corbin A. Use of Bacillus consortium in waste digestion and pathogen control in shrimp aquaculture. International Biodeterioration & Biodegradation, 2015; 102(1): 159–164.
[5] Shao Y L, Zhong H, Chen L H. Microbiologic technology for purifying coastal aquaculture water. Fresenius Environmental Bulletin, 2018; 27: 3796–3802.
[6] Wang L, Shao X, Xu M, Chen S. Bioremediation of nitrogen- and phosphorus-polluted aquaculture sediment by utilizing combined immobilized effective microorganisms and sediment aeration technology. Inter J Agric & Biol Eng, 2019; 12(6): 192–201.
[7] Fan L, Chen J, Liu Q, Wu W, Meng S, Song C, et al. Exploration of three heterotrophic nitrifying strains from a tilapia pond for their characteristics of inorganic nitrogen use and application in aquaculture water. Journal of Bioscience & Bioengineering, 2015; 119(3): 303–309.
[8] Travaini-Lima F, Andreia M S D V M, Sipauba-Tavares L J. Constructed wetland for treating effluent from subtropical aquaculture farm. Water Air & Soil Pollution, 2015; 226(3): 1–10.
[9] Zhang X, Hu J, Spanjers H, van Lier J B. Performance of inorganic coagulants in treatment of backwash waters from a brackish aquaculture recirculation system and digestibility of salty sludge. Aquacultural Engineering, 2014; 61(3): 9–16.
[10] Tomasa D C C M, Rodríguez R A, Voltolina D, Morquecho L. Effectiveness of coagulants-flocculants for removing cells and toxins of Gymnodinium catenatum. Aquaculture, 2016; 21(2): 678–688.
[11] Li G, Lu Z, Zhang J, Li H, Zhou Y, Mohammed I Z A, et al. Life cycle assessment of biofuel production from microalgae cultivated in anaerobic digested wastewater. Inter J Agric & Biol Eng, 2020; 13(1): 241–246.
[12] Okaiyeto K, Nwodo U U, Mabinya L V, Okoh A I. Evaluation of the flocculation potential and characterization of bioflocculant produced by Micrococcus sp. Leo. Applied Biochemistry & Microbiology, 2014; 50(6): 601–608.
[13] Salehizadeh H, Yan N. Recent advances in extracellular biopolymer flocculants. Biotechnology Advances, 2014; 32(8): 1506–1522.
[14] Ugbenyen A M, Cosa S, Mabinya L V, Okoh A I. Bioflocculant production by Bacillus sp. Gilbert isolated from a marine environment in South Africa. Applied Biochemistry & Microbiology, 2014; 50(1): 49–54.
[15] Gong W X, Wang S G, Sun X F, Liu X W, Yue Q Y, Gao B Y. Bioflocculant production by culture of Serratia ficaria and its application in wastewater treatment. Bioresource Technology, 2008; 99(11): 4668–4674.
[16] Li G, Zhang J, Li H, Hu R, Yao X, Liu Y, et al. Towards high-quality biodiesel production from microalgae using original and anaerobically-digested livestock wastewater. Chemosphere, 2020; 4: 128578.
[17] Kim D G, La H J, Ahn C Y, Park Y H, Oh H M. Harvest of Scenedesmus sp. with bioflocculant and reuse of culture medium for subsequent high-density cultures. Bioresource Technology, 2011; 102(3): 3163–3168.
[18] Zhao H, Liu H, Zhou J. Characterization of a bioflocculant MBF-5 by Klebsiella pneumoniae and its application in Acanthamoeba cysts removal. Bioresource Technology, 2013; 137(3): 226–232.
[19] Chen L H, Huang X Q, Zhang F G, Zhao D K, Yang X M, Shen Q R. Application of Trichoderma harzianum SQR-T037 bio-organic fertiliser significantly controls Fusarium wilt and affects the microbial communities of continuously cropped soil of cucumber. Journal of the Science of Food and Agriculture, 2012; 92(12): 2465–2470.
[20] Ghosh M, Ganguli A, Pathak S. Application of a novel biopolymer for removal of Salmonella from poultry wastewater. Environmental Technology, 2009; 30(4): 337–344.
[21] Lee G C, Jheong W H, Kim M J, Choi D H, Baik K H. A 5-year survey (2007–2011) of enteric viruses in Korean aquatic environments and the use of coliforms as viral indicators. Microbiology and Immunology, 2013; 57(1): 46–53.
[22] Kriem M R, Banni B, Bouchtaoui H E, Hamama A, Quilici M L. Prevalence of Vibrio spp. in raw shrimps (Parapenaeus longirostris) and performance of a chromogenic medium for the isolation of Vibrio strains. Letters in Applied Microbiology, 2015; 61(3): 231–240.
[23] Ruxton T, Graeme D, Beauchamp Y, Guy Y K. Time for some a priori thinking about post hoc testing. Behavioral Ecology, 2008; 3(1): 161–172.
[24] Lertsutthiwong P, Sutti S, Powtongsook S. Optimization of chitosan flocculation for phytoplankton removal in shrimp culture ponds. Aquacultural Engineering, 2009; 41(3): 188–193.
[25] De-Bashan L E, Trejo A, Huss V A R, Hernandez J P, Bashan Y. Chlorella sorokiniana UTEX 2805, a heat and intense, sunlight-tolerant microalga with potential for removing ammonium from wastewater. Bioresource Technology, 2008; 99(11): 4980–4989.
[26] Bajaj I, Singhal R. Poly (glutamic acid)- an emerging biopolymer of commercial interest. Bioresource Technology, 2011; 102(10): 5551–5561.
[27] Wang N, Yang G, Che Y, Liu C. Heterogenous expression of poly-γ-glutamic acid synthetase complex gene of Bacillus licheniformis WBL-3. Applied Biochemistry & Microbiology, 2011; 5(2): 54–61.
[28] Wu Q, Ni M, Dou K, Tang J, Ren J, Yu C, et al. Co-culture of Bacillus amyloliquefaciens ACCC11060 and Trichoderma asperellum GDFS1009 enhanced pathogen-inhibition and amino acid yield. Microbial Cell Factories, 2018; 17(1): 347–356.
[29] Li G, Ji F, Bai X, Zhou Y, Dong R, Huang Z. Comparative study on thermal cracking characteristics and bio-oil production from different microalgae using Py-GC/MS. Inter J Agric & Biol Eng, 2019; 12(1): 208–213.
[30] Thanigaivel S, Vijayakumar S, Mukherjee A, Chandrasekaran N, Thomas J. Antioxidant and antibacterial activity of Chaetomorpha antennina against shrimp pathogen Vibrio parahaemolyticus. Aquaculture, 2014; 433: 467–475.
[31] Li G, Bai X, Li H, Lu Z, Zhou Y, Wang Y, et al. Nutrients removal and biomass production from anaerobic digested effluent by microalgae: A review. Inter J Agric & Biol Eng, 2019; 12(5): 8–13.
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Published
2021-04-03
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Shao, Y., Zhong, H., Wang, L., & Elbashier, M. M. (2021). Bacillus amyloliquefaciens (IAE635) and their metabolites could purify pollutants, Vibrio spp. and coliform bacteria in coastal aquaculture wastewater. International Journal of Agricultural and Biological Engineering, 14(2), 205–210. Retrieved from https://ijabe.migration.pkpps03.publicknowledgeproject.org/index.php/ijabe/article/view/6082
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Biosystems, Biological and Ecological Engineering
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