Optimizing a nitrogen-supplemented, condensed corn soluble medium for growth of the Polyhydroxyalkanoate producer Pseudomonas putida KT217
Keywords:
cell density, cell dry weight (CDW), biodegradable plastic, biocreator, pH, nitrogen supplement, growth rateAbstract
Pseudomonas putida KT217 produces medium-chain-length polyhydroxyalkanoate (mcl-PHA) that is of commercial interest as a biodegradable plastic. To reduce PHA production costs, a less expensive medium to grow P. putida KT217 to a high cell dry weight (CDW) was developed. P. putida KT217 was grown in aerated shake flasks on a condensed corn solubles (CCS) based medium that provided several organic acids and carbohydrates that were utilized for growth. The medium was prepared by adding various amounts of fresh CCS (100-600 g/L, or 34.9-209.4 g/L dry basis) to water and then centrifuging and filtering. The CCS permeate media contained dry matter levels of 28.8-164.9 g/L. The CCS permeate medium containing 108.4 g/L solids produced approximately 6 g CDW/L, at a growth rate of 1.03 per hour, and maximum cell population of 4References
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[3] Hejazi P, Vasheghani-Farahani E, Yamini Y. Supercritical fluid disruption of Ralstonia eutropha for poly (B-hydroxybutyrate) recovery. Biotechnology Progress, 2003; 19: 1519-1523.
[4] Yu P H F, Chua H, Huang A L, Lo W H, Ho K P. Transformation of industrial food wastes into polyhydroxyalkanoates. Water Science and Technology, 1999; 40(1): 365-370.
[5] Yu J. Production of PHA from starchy wastewater via organic acids. Journal of Biotechnology, 2001; 86: 105- 112.
[6] Koller M, Bona R, Braunegg G, Hermann C, Horvat P, Kroutil M, et al. Production of polyhydroxyalkanoates from agricultural waste and surplus materials. Biomacromolecules, 2005; 6: 561-565.
[7] Ribera R G, Monteoliva-Sanchez M, Ramos-Cormenzana A. Production of polyhidroxyalkanoates by Pseudomonas putida KT2442 harboring pSK2665 in wastewater from olive oil mills (alpechin). Electronic Journal of Biotechnology, 2001; 4(2): 116-119.
[8] Vidal-Mas J, Resina-Pelfort O, Haba E, Comas J, Manresa A, Vives-Rego J. Rapid flow cytometry ? Nile red assessment of PHA cellular content and heterogeneity in cultures of Pseudomonas aeruginosa 47T2 (NCIB 40044) grown in waste frying oil. Antoine van Leeuwenhoek, 2001; 80: 57-63.
[9] Du G, Yu J. Green technology for conversion of food scraps to biodegradeable thermoplastic polyhydroxyalkanoates. Environmental Science and Technology, 2002; 36: 5511-5516.
[10] Du G, Chen L X L, Yu J. High-efficiency production of bioplastics from biodegradeable organic solids. Journal of Polymers and the Environment, 2004; 12(2): 89-94.
[11] Keenan T M, Tanenbaum S W, Stipanovic A J, Nakas J P. Production and characterization of poly-B-hydroxyalkanoate copolymers from Burkholderia cepacia utilizing xylose and levulinic acid. Biotechnology Progress, 2004; 20: 1697- 1704.
[12] Gouda M K, Sellam A E, Omar S H. Production of PHB by a Bacillus megaterium strain usin gsugarcane molasses and corn steep liquor as sole carbon and nitrogen sources. Microbiological Research, 2001; 156: 201-207.
[13] Purushothaman M, Anderson R K I, Narayana S, Jayaraman V K. Industrial byproducts as cheaper medium components influencing the production of polyhydroxyalkanoates (PHA)-biodegradeable plastics. Bioprocess and Biosystem Engineering, 2001; 24: 131-136.
[14] Bock S A, Fox S L, Gibbons W R. Development of a low cost, industrially suitable medium for production of acetic acid from glucose by Clostridium thermoaceticum. Biotechnology and Applied Biochemistry, 1997; 25: 117- 125.
[15] Fosmer A, Gibbons W R, Heisel N. Scleroglucan production from Sclerotium glucanicum on a condensed corn solubles medium. Journal of Biotechnology Research, 2010; 2: 131-143.
[16] Smith R L, West T P, Gibbons W R. Rhodospirillum rubrum: Utilization of condensed corn solubles for poly-(3- hydroxybutyrate-co-3-hydroxyvalerate) production. Journal of Applied Microbiology, 2008; 104: 1488-1494.
[17] Vanderhoff A, Gibbons W R, Bauer N, West T P. Development of a low-cost medium for producing gellan from Sphingomonas paucimobilis. Journal of Biotech Research, 2010; 2: 67-78.
[18] Wolf-Hall C E, Gibbons W R, Bauer N A. Development of a low-cost medium for production of nisin from Lactococcus lactis subsp. lactis. World Journal of Microbiology and Biotechnology, 2009; 25: 2013-2019.
[19] Tan I K P, Kumar K S, Theanmalar M, Gan S N, Gordon III B. Saponified palm kernel oil and its major free fatty acids as carbon substrates for the production of polyhydroxyalkanoates in Pseudomonas putida PGA1. Applied Microbiology Biotechnology, 1997; 47: 207-211.
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Published
2012-12-11
How to Cite
Javers, J., Gibbons, W., & Karunanithy, C. (2012). Optimizing a nitrogen-supplemented, condensed corn soluble medium for growth of the Polyhydroxyalkanoate producer Pseudomonas putida KT217. International Journal of Agricultural and Biological Engineering, 5(4), 62–67. Retrieved from https://ijabe.migration.pkpps03.publicknowledgeproject.org/index.php/ijabe/article/view/607
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Renewable Energy and Material Systems
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