Photosynthetic profiling of a Dunaliella salina mutant DS240G-1 with improved β-carotene productivity induced by heavy ions irradiation
Keywords:
microalgae, D. salina, carbon-ions irradiation, chlorophyll fluorescence, β-caroteneAbstract
Carbon-ion irradiation is a technique for trait improvement in the microalgae, but the underlying mechanisms that how it altered the biomass, and photosynthetic pigments accumulation were unclear. One mutant (DS240G-1) was obtained from Dunaliella salina by heavy ion irradiation mutagenesis. Compared to the wild type, the biomass accumulation and maximum growth rate of DS240G-1 were increased by 34% and 55% respectively, and its β-carotene content was 21% higher than the wild type. Subsequent analysis of the chlorophyll fluorescence parameters indicated that higher β-carotene productivity was likely owing to the improved maximum quantum efficiency (Fv/Fm) and decreased thermal dissipation of photosynthesis in DS240G-1 than that of wild type during cultivation. In addition, the result of this study revealed that high content of ROS may induce β-carotene accumulation in mutant DS240G-1. Also, the total fatty acid (TFA) content in mutant DS240G-1 was 79% higher than that in wild type. Owing to its high β-carotene productivity and total fatty acid content, DS240G-1 could be considered as a promising candidate for microalgae β-carotene and biodiesel production. This work provided the first insight into the biological effects involved in carbon-ions irradiation on the photosynthetic activity of D. salina. Keywords: microalgae, D. salina, carbon-ions irradiation, chlorophyll fluorescence, β-carotene DOI: 10.25165/j.ijabe.20211402.5993 Citation: Xi Y M, Liu Y, Chi Z Y, Yin L, Wang L J, Luo G H. Photosynthetic profiling of a Dunaliella salina mutant DS240G-1 with improved β-carotene productivity induced by heavy ions irradiation. Int J Agric & Biol Eng, 2021; 14(2): 211–217.References
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[2] Li G, Bai X, Huo S H, Huang Z G. Fast pyrolysis of LERDADEs for renewable biofuels. IET Renewable Power Generation, 2020; 14(6): 959–967.
[3] Li G, Zhang J, Li H, Hu R C, Yao X L, Liu Y, et al. Towards high-quality biodiesel production from microalgae using original and anaerobically-digested livestock wastewater. Chemosphere, 2020; 128578.
[4] Li G, Lu Z T, Zhang J, Li H, Zhou Y G, Zayan A M I, et al. Life cycle assessment of biofuel production from microalgae cultivated in anaerobic digested wastewater. Int J Agric & Biol Eng, 2020; 13(1): 241–246.
[5] Mojaat M, Pruvost J, Foucault A, Legrand J. Effect of organic carbon sources and Fe2+ ions on growth and beta-carotene accumulation by Dunaliella salina. Biochemical Engineering Journal, 2008; 39: 177–184.
[6] Tafreshi A H, Shariati M. Dunaliella biotechnology: Methods and applications. Journal of Applied Microbiology, 2009; 107: 14–35.
[7] Shaish A, Benamotz A, Avron M. Production and selection of high beta-carotene mutants of Dunaliella-bardawil (chlorophyta). Journal of Phycology, 1991; 27: 652–656.
[8] Mogedas B, Casal C, Forjan E, Vilchez C. Beta-Carotene production enhancement by UV-A radiation in Dunaliella bardawil cultivated in laboratory reactors. Journal of Bioscience And Bioengineering, 2009; 108: 47–51.
[9] Gong M Y, Bassi A. Carotenoids from microalgae: A review of recent developments. Biotechnology Advances, 2016; 34: 1396–1412.
[10] Jin E S, Feth B, Melis A. A mutant of the green alga Dunaliella salina constitutively accumulates zeaxanthin under all growth conditions. Biotech-nology and Bioengineering, 2003; 81: 115–124.
[11] Ma Y, Wang Z, Zhu M, Yu C, Cao Y, Zhang D, et al. Increased lipid productivity and TAG content in Nannochloropsis by heavy-ion irradiation mutagenesis. Bioresource Technology, 2013; 136: 360–367.
[12] Kamalanathan M, Ly Hai Thi D, Chaisutyakorna P, Gleadow R, Beardall J. Photosynthetic physiology of Scenedesmus sp. (Chlorophyceae) under photoautotrophic and molasses-based heterotrophic and mixotrophic conditions. Phycologia, 2017; 56: 666–674.
[13] Hu G, Fan Y, Zhang L, Yuan C, Wang J, Li W, et al. Enhanced lipid productivity and photosynthesis efficiency in a Desmodesmus sp. mutant induced by heavy carbon ions. Plos One, 2013; 8: 1–11.
[14] Li G, Bai X, Li H, Lu Z T, Zhou Y G, Wang Y K, et al. Nutrients removal and biomass production from anaerobic digested effluent by microalgae: A review. Int J Agric & Biol Eng, 2019; 12(5): 8–13.
[15] Wang J, Li X, Lu D, Du Y, Ma L, Li W, et al. Photosynthetic effect in Selenastrum capricornutum progeny after carbon-ion irradiation. Plos One, 2016; 11: 1–11.
[16] Lamers P P, Janssen M, de Vos R C H, Bino R J, Wijffels R H. Carotenoid and fatty acid metabolism in nitrogen-starved Dunaliella salina, a unicellular green microalga. Journal of Biotechnology, 2012; 162: 21–27.
[17] Cao X P, Xi Y M, Liu J, Chu Y D, Wu P C, Yang M, et al. New insights into the CO2-steady and pH-steady cultivations of two microalgae based on continuous online parameter monitoring. Algal Research-Biomass Biofuels and Bioproducts, 2019; 38: 1–6.
[18] Liu J, Liu Y, Wang H, Xue S. Direct transesterification of fresh microalgal cells. Bioresource Technology, 2015; 176: 284–287.
[19] Sui Y X, Muys M, Vermeir P, D'Adamo S, Vlaeminck S E. Light regime and growth phase affect the microalgal production of protein quantity and quality with Dunaliella salina. Bioresource Technology, 2019; 275: 145–152.
[20] Zheng Z B, Gao S, He Y, Li Z Y, Li Y X, Cai X H, et al. The enhancement of the oxidative pentose phosphate pathway maybe involved in resolving imbalance between photosystem I and II in Dunaliella salina. Algal Research-Biomass Biofuels and Bioproducts, 2017; 26: 402–408.
[21] Zhu C B, Zhai X Q, Jia J, Wang J H, Han D S, Li Y H, et al. Seawater desalination concentrate for cultivation of Dunaliella salina with floating photobioreactor to produce beta-carotene. Algal Research-Biomass Biofuels and Bioproducts, 2018; 35: 319–324.
[22] Yao C H, Ai J N, Cao X P, Xue S, Zhang W. Enhancing starch production of a marine green microalga Tetraselmis subcordiformis through nutrient limitation. Bioresource Technology, 2012; 118: 438–444.
[23] Williams P L, Laurens L L. Microalgae as biodiesel & biomass feedstocks: Review & analysis of the biochemistry, energetics & economics. Energy & Environmental Science, 2010; 3: 554–590.
[24] Qiang H, Zarmi Y, Richmond A. Combined effects of light intensity, light-path and culture density on output rate of Spirulina platensis (Cyanobacteria). European Journal of Phycology, 1998; 33: 165–171.
[25] Manandhar-Shrestha K, Hildebrand M. Development of flow cytometric procedures for the efficient isolation of improved lipid accumulation mutants in a Chlorella sp microalga. Journal of Applied Phycology, 2013, 25: 1643–1651.
[26] Anandarajah K, Mahendraperumal G, Sommerfeld M, Hu Q. Characterization of microalga Nannochloropsis sp. mutants for improved production of biofuels. Applied Energy, 2012; 96: 371–377.
[27] White S, Anandraj A, Bux F. PAM fluorometry as a tool to assess microalgal nutrient stress and monitor cellular neutral lipids. Bioresource Technology, 2011; 102: 1675–1682.
[28] Havlik I, Reardon K F, Uenal M, Lindner P, Prediger A, Babitzky A, et al. Monitoring of microalgal cultivations with on-line, flow-through microscopy. Algal Research-Biomass Biofuels and Bioproducts, 2013; 2: 253–257.
[29] Obata M, Toda T, Taguchi S. Using chlorophyll fluorescence to monitor yields of microalgal production. Journal of Applied Phycology, 2009; 21: 315–319.
[30] MacIntyre H L, Kana T M, Anning T, Geider R J. Photoacclimation of photosynthesis irradiance response curves and photosynthetic pigments in microalgae and cyanobacteria. Journal of Phycology, 2002; 38: 17–38.
[31] Xu Y, Harvey P J. Carotenoid Production by Dunaliella salina under Red Light. Antioxidants, 2019; 8: 1–11.
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Published
2021-04-03
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Xi, Y., Liu, Y., Chi, Z., Yin, L., Wang, L., & Luo, G. (2021). Photosynthetic profiling of a Dunaliella salina mutant DS240G-1 with improved β-carotene productivity induced by heavy ions irradiation. International Journal of Agricultural and Biological Engineering, 14(2), 211–217. Retrieved from https://ijabe.migration.pkpps03.publicknowledgeproject.org/index.php/ijabe/article/view/5993
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