Microbiological biostimulant promotes rooting of olive cuttings
Keywords:
Azospirillum brasilense, Arbuscular mycorrhizal fungi, Propagation, Olea europaea L., microorganismAbstract
The most widely used method of olive propagation is cutting, although for some cultivars the technique is still limited. The objective of this work was to evaluate the use of plant growth promoter bacteria and arbuscular mycorrhizal fungi in the rooting of olive cuttings. Two experiments were conducted from October 2017 to January 2018 with cuttings from 4 olive cultivars (Ascolano 315, Koroneiki, Maria da Fé and Picual) from four-year-old plants. The first experiment consisted of 4 olive cultivars and 3 mycorrhizal species plus the control and the second experiment of 3 concentrations of commercial inoculant (Nitro1000®), containing Azospirillum brasilense plus control. The cuttings were treated with 3000 mg/L of indolebutyric acid (IBA). The experimental design used in the two experiments was randomized blocks, in a 4 × 4 factorial scheme, containing 25 stakes per replicate and 3 replicates. The cuttings were kept under weaver conditions and within a low rooting tunnel. After 75 d, the percentage of rooted cuttings, callus and sprouts, length of the largest root, number and length of shoots were evaluated. The use of FMA in Maria da Fé and Picual has a positive response to cutting rooting and the fungi benefit the quality of the seedlings by the higher number of shoots and larger root length. The relation of Azospirillum brasilense cultivar is specific, being that, the cultivar Picual does not respond to inoculation of the bacterium having less viability in the propagation by cutting. Maria da Fé and Koroneiki have the best percentages of rooting with the use of 75.00 and 52.00 mL/L of inoculant, respectively. Keywords: Azospirillum brasilense, arbuscular mycorrhizal fungi, propagation, Olea europaea L., microorganism DOI: 10.25165/j.ijabe.20211406.5880 Citation: Ritter G, Villa F, da Silva D F, Alberton O, Menegusso F J, Eberling T, et al. Microbiological biostimulant promotes rooting of olive cuttings. Int J Agric & Biol Eng, 2021; 14(6): 207–212.References
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[30] Silva T F, Melloni R, Melloni E G P, Gonçalves E D. Non-symbiotic diazotrophic bacteria and the rooting of olive semi-hardwood cuttings (Olea europaea L.). Ciênc. Florest, 2017; 27(1): 67–71.
[31] Oliveira M C, Ramos J D, Pio R, Dos Santos V A, Silva F O R. Cutting rooting of promising olive cultivars for the Mantiqueira Range. Rev. Ceres, 2012; 59(1): 147–150.
[32] Hartmann H T, Kester D E, Davies J R F T, Geneve R L, Wilson S B. Plant Propagation: Principles and Practices. Pearson, 9 Ed., 2017; 1024p.
[33] Vignolo G K, Picolotto L, Gonçalves M A, Pereira I S, Antunes L E C. Presence of leaves on rooting of blackberry. Ciênc. Rur, 2014; 44(3): 467–472.
[34] Lima R L S, Siqueira D L, Weber O B, Cazetta J O. Length of cuttings and part of the branch in the formation of saplings. Rev.Bras Frut, 2006; 28(1): 83–86.
[35] Picolotto L, Vignolo G K, Pereira I S, Goncalves M A, Antunes L E C. Rooting of blackberry cuttings in function of fertilization nitrogen in the mother plant. Rev. Ceres. 2015; 62(3): 294–300.
[36] Montero-Calasanz M C, Santamaría C, Albareda M, Daza A, Duan J. Alternative rooting induction of semi-hardwood olive cuttings by several auxin-producing bacteria for organic agriculture systems. Span. J. Agric. Res, 2013; 11(1): 146–154.
[37] Quadros P D, Roesch L F W, Silva P R F, Vieira V M, Roehrs D D. Field agronomic performance of maize hybrids inoculated with Azospirillum Rev. Ceres, 2014; 61(2): 209–218.
[2] Fachinello J C, Hoffmann A, Nachtigal J C. Fruit plants propagation. Brasília, 2011; 221p.
[3] Smarsi R C, Chagas E A, Reis L L, Oliveira G F, Mendonça V. Concentrations of indolebutyric acid and kinds of substrates in the vegetative propagation of litchi. Rev. Bras. Fruit, 2008; 30(1): 7–11.
[4] Mohamed Y I. Evaluation the effect of rooting media and hormonal concentrations (IBA) in three olive cultivars growing in SiwaOesis-Egypt. International J. Agric Sci, 2015; 5(3): 500–503.
[5] Hechmi M, Khaled M, Abed S, El-Hassen A, Faiez R. Performance of olive cuttings (Olea europaea L.) of different cultivars growing in the agro-climatic conditions of Al-Jouf (Saudi Arabia). Am. J. Plant Physiol, 2013; 8(1): 41–49.
[6] Penso G A, Sachet M R, Maro L A C, Patto L S, Citadin I. Spread of ‘Koroneiki’ Olive by the cutting method at different times, AIB concentrations and the presence of leaves. Revista Ceres, 2016; 63(3): 355–360.
[7] Villa F, Silva D F, Dall’oglio P, Potrich C, Menegusso F J. Performance of substrates in rooting capacity of olive tree cuttings. Ver. Ciênc. Agrovet, 2017; 16(2): 95–101.
[8] Gai J, Gao W, Liu L, Chen Q, Feng G. Infectivity and community composition of arbuscular mycorrhizal fungi from different soil depths in intensively managed agricultural ecosystems. J. Soils Sed, 2015; 15(5): 1–12.
[9] Kachkouch W, Touhami A O, Filali-Maltouf A, Modafar C, Moukhli A. Arbuscular mycorrhizal fungi species associated with rhizosphere of Olea europaea L. in Morocco. J. An. Plant Sci, 2012; 15(3): 2275–2287.
[10] Bompadre M J, Pérgola M, Fernández Bidondo L, Colombo RP, Silvani VA. Evaluation of arbus-cular mycorrhizal fungi capacity to alleviate abiotic stress of olive (Olea europaeaL.) plants at different transplant conditions. The Sci. World J, 2014; 1(1): 1–13.
[11] Silva F J, Ribeiro R C F, Xavier A A, Santos Neto J A, Souza M A. Rhizobacteria associated with organic materials in the control of root-knot nematode in tomato. Hort. Bras, 2016; 34(1): 59–65.
[12] Vejan P, Abdullah R, Khadiran T, Ismail S, Nasrulhaq Boyce A. Role of plant growth promoting rhizobacteria in agricultural sustainability. Molec, 2016; 21(5): 573.
[13] Masciarelli O, Urbani L, Reinoso H, Luna V. Alternative mechanism for the evaluation of indole-3-acetic acid (IAA) production by Azospirillum brasilense strains and its effects on the germination and growth of maize seedlings. J. Microb, 2013; 51(5): 590–597.
[14] Fukami J, Nogueira M A, Araujo R S, Hungria M. Accessing inoculation methods of maize and wheat with Azospirillum brasilense. AMB Express, 2016; 6(1): 3. doi: 10.1186/s13568-015-0171-y
[15] Li G, Bai X, Li H, Lu Z T, Zhou Y G, Wang Y K, et al. Nutrients removal and biomass production from anaerobic digested effluent by microalgae: A review. Int J Agric & Biol Eng, 2019; 12(5): 8–13.
[16] Li G, Zhang J, Li H, Hu R C, Yao X L, Liu Y, et al. Towards high-quality biodiesel production from microalgae using original and anaerobically-digested livestock wastewater. Chemosphere, 2021; 273: 128578. doi: 10.1016/j.chemosphere.2020.128578.
[17] Alvarez C A, Stape J L, Sentelhas P C, Gonçalves J L M, Sparovek G. Koppen’s climate classification map for Brazil. Meteorol. Zeitschrift, 2013; 22(6): 711–728.
[18] Caviglione J H, Kiikl L R, Aramori P H, Oliveira D. Climate charts from
Paraná: IAPAR, 2000.
[19] Oliveira M C, Vieira Neto J, Oliveira R S, Pio R, Oliveira N C. Rooting of cuttings of two olive cultivars submitted to the application of different fertilizers. Brag, 2010; 69(1): 99–103.
[20] Ferreira D F. SISVAR: A computer statistical analysis system. Ciênc. Agrotec, 2011; 35 (6): 1039–1042.
[21] Pio R, Costa F C, Curi P N, Moura P H M. Rooting of kiwi cultivars woody cuttings. Scientia Agraria, 2010; 11(3): 271–274.
[22] Vieira V C S, Melloni R, Vieira Neto J. Assessment of mycorrhizal interaction in olive tree (Olea europaea L.). Ver. Bras. Ciênc. Solo, 2011; 35(6): 1885–1892.
[23] Chatzistathis T, Orfanoudakis M, Alifragis D, Therios I. Colonization of Greek olive cultivars root system by arbuscular mycorrhizal fungus: Root morphology, growth, and mineral nutrition of olive plants. Scient. Agric, 2013; 70(1): 185–94.
[24] Ferreira G M R, Melloni R, Silva L F O, Martins F M, Gonçalves E D. Arbuscular mycorrhizal fungi in the development of olive tree seedlings (Olea europaea L.) Cultivated in southern Minas Gerais. Rev. Bras. Ciênc. Solo, 2015; 39(1): 361–366.
[25] Oliveira A F, Chalfun N N J, Alvarenga A A, Vieira Neto J, Pio R. Olive cuttings at different times, substrates and doses of IBA diluted in NaOH and alcohol. Ciênc. Agrotec, 2009; 33(1): 79–85.
[26] Rosa G G, Zanandrea I, Mayer N A, Bianchi V J. Propagation of Prunus spp. rootstocks by cutting: effects of genotype, branch developmental stage, and cutting type. Revista Ceres, 2017; 64(1): 90–97.
[27] Mohan J E, Cowden C C, Baas P, Dawadi A, Frankson P T. Mycorrhizal fungi mediation of terrestrial ecosystem responses to global change: mini-review. Fungal Ecol, 2014; 10(1): 3–19.
[28] Taiz L, Zeiger E, Moller I M, Murphy A. Physyology and plant development. 6. ed. Porto Alegre: Artmed, 2017; 858 p.
[29] Séry D J, Kouadio Z G, Voko B R, Zézé A. Selecting native arbuscular mycorrhizal fungi to promote cassava growth and increase yield under Field conditions. Front. Microbiol, 2016; 22(7): 2063–2074.
[30] Silva T F, Melloni R, Melloni E G P, Gonçalves E D. Non-symbiotic diazotrophic bacteria and the rooting of olive semi-hardwood cuttings (Olea europaea L.). Ciênc. Florest, 2017; 27(1): 67–71.
[31] Oliveira M C, Ramos J D, Pio R, Dos Santos V A, Silva F O R. Cutting rooting of promising olive cultivars for the Mantiqueira Range. Rev. Ceres, 2012; 59(1): 147–150.
[32] Hartmann H T, Kester D E, Davies J R F T, Geneve R L, Wilson S B. Plant Propagation: Principles and Practices. Pearson, 9 Ed., 2017; 1024p.
[33] Vignolo G K, Picolotto L, Gonçalves M A, Pereira I S, Antunes L E C. Presence of leaves on rooting of blackberry. Ciênc. Rur, 2014; 44(3): 467–472.
[34] Lima R L S, Siqueira D L, Weber O B, Cazetta J O. Length of cuttings and part of the branch in the formation of saplings. Rev.Bras Frut, 2006; 28(1): 83–86.
[35] Picolotto L, Vignolo G K, Pereira I S, Goncalves M A, Antunes L E C. Rooting of blackberry cuttings in function of fertilization nitrogen in the mother plant. Rev. Ceres. 2015; 62(3): 294–300.
[36] Montero-Calasanz M C, Santamaría C, Albareda M, Daza A, Duan J. Alternative rooting induction of semi-hardwood olive cuttings by several auxin-producing bacteria for organic agriculture systems. Span. J. Agric. Res, 2013; 11(1): 146–154.
[37] Quadros P D, Roesch L F W, Silva P R F, Vieira V M, Roehrs D D. Field agronomic performance of maize hybrids inoculated with Azospirillum Rev. Ceres, 2014; 61(2): 209–218.
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2021-12-16
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Ritter, G., Villa, F., da Silva, D. F., Alberton, O., Menegusso, F. J., Eberling, T., & Dória, J. (2021). Microbiological biostimulant promotes rooting of olive cuttings. International Journal of Agricultural and Biological Engineering, 14(6), 207–212. Retrieved from https://ijabe.migration.pkpps03.publicknowledgeproject.org/index.php/ijabe/article/view/5880
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Renewable Energy and Material Systems
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