Screening and performance of L-14, a novel, highly efficient and low temperature-resistant cellulose-degrading strain
Keywords:
low-temperature-resistant, Neurospora sp., 18S rDNA sequence, carboxymethyl cellulase, response surface methodologyAbstract
In view of the low bioconversion efficiency of agricultural biomass waste in low-temperature environments in winter, a low-temperature-resistant cellulose-degrading strain, L-14, was successfully screened by restrictive cultures from humus-rich soil in the Daqing Zhalong wetland region. According to morphological observations and 18S rDNA sequence analysis, the cellulose-degrading strain L-14 was identified as a Neurospora sp, belonging to fungus. Different parameters, such as temperature, initial pH, carbon, nitrogen and lecithin, were optimized using a single-factor experiment and a response surface methodology (RSM). When the temperature was 16°C, the optimal conditions for enzyme production were an initial pH 8.20, 10.45 g/L of bran, 5.28 g/L of yeast powder, and 4.25 g/L of lecithin. The carboxymethyl cellulase (CMCase) activity of strain L-14 was 63.598 IU/mL. Strain L-14 had a high level of cellulose degradation activity, excellent resistance to low temperatures and environmental adaptability, indicating its good application prospects in substrates pretreatment of biogas engineering. Keywords: low-temperature-resistant, Neurospora sp., 18S rDNA sequence, carboxymethyl cellulase, response surface methodology DOI: 10.25165/j.ijabe.20201301.5128 Citation: Zheng G X, Lu Z X, Li J, Ai S, Sun Y. Screening and performance of L-14, a novel, highly efficient and low temperature-resistant cellulose-degrading strain. Int J Agric & Biol Eng, 2020; 13(1): 247–254.References
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[3] Wang Q, Li S N, Li H Y, Lei B S, Chen Y, Zhang L J, et al. Screening and identification on capable of degrading cellulose of produce spore cellulose decomposing bacteria xn-13. Chinese Agricultural Science Bulletin, 2009; 25(11): 180–185. (in Chinese)
[4] Zhao G, Ma F, Wei L, Cai H, Wang Z. Research and prospects of low temperature biogas digestion technology in North. Journal of Harbin Institute of Technology, 2011; 43(6): 29–33. (in Chinese)
[5] Saloheimo M. Swollenin, a trichoderma reesei protein with sequence similarity to the plant expansins, exhibits disruption activity on cellulosic materials. Eur. J. Biochem., 2002; 269(17): 4202–4211.
[6] Hamelinck C N, Hooijdonk G., Faaij A P C. Ethanol from lignocellulosic biomass: techno-economic performance in short-, middle- and long-term. Biomass Bioenerg, 2005; 28: 384–410.
[7] Zhang X F, Yao T D, Tian L D, Xu S J, An L Z. Phylogenetic and physiological diversity of bacteria isolated from puruogangri ice core. Microbial Ecology, 2008; 55(3): 476–488.
[8] Feller U. Moderately high temperatures inhibit ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) activase-mediated activation of rubisco. Plant Physiology, 1998; 116(2): 539–546.
[9] Jarvinen K T, Melin E S, Puhakka J A. High-rate bioremediation of chlorophenol-contaminated groundwater at low temperatures. Environmental Science & Technology, 1994; 28(13): 2387–2392.
[10] Li X M, Jia B X. Isolation and identification of an equol-producing bacterial strain from vegetarian intestinal tract. Food Science, 2014; 35(3): 153–156. (in Chinese)
[11] Li C Y, Xu C H, Pan J B. Isolation of a Chlorimuron-Ethyl Degradation Bacterium and Its Bioremediation in Contamination Soil. International Conference on Bioinformatics & Biomedical Engineering. IEEE. 2011. doi: 10.1109/icbbe.2011.5780302
[12] Kumar S, Nei M, Dudley J, Tamura K. Mega: a biologist-centric software for evolutionary analysis of dna and protein sequences. Briefings in Bioinformatics, 2008; 9(4): 299–306.
[13] Zhao Z Y, Huang Q F, Wang Q. Research progress on the degradation of deca-bdes. Research of Environmental Sciences, 2014; 27(5): 554–559.
[14] Teng G S, Liu Y, Wu L D, Fan C Y, Wang J Z, Guan B, et al. Optimization of l-lysine medium by response surface methodology. Food & Machinery, 2015; 31(5): 256–260. (in Chinese)
[15] Zhang W, Zhang Q, Ruan X Y, Wang X Z, Kong H N, Lin Y. Optimization of enzymatic hydrolysis of wheat straw by using response surface methodology. Biomass Chemical Engineering, 2015; 49(2): 39–46. (in Chinese)
[16] Liu S, Fan B Q. Screening of a straw cellulose-degrading fungi qsh3-3 and study on its characteristics of cellulase production. Plant Nutrition & Fertilizer Science, 2012; 18(1): 218–226.
[17] Zhang C M. Screening of bacteria decomposing low-temperature cellulose and the application of composite microbial inoculants in straw returning to field. MS dissertation, Nanjing Agriculture University, 2014. (in Chinese)
[18] Chen L. Breeding and fermentation medium optimization of cold-active cellulase strain CNY086(I). Microbiology, 2009; 36(10): 1547–1552. (in Chinese)
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Published
2020-03-02
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Zheng, G., Lu, Z., Li, J., & Ai, S. (2020). Screening and performance of L-14, a novel, highly efficient and low temperature-resistant cellulose-degrading strain. International Journal of Agricultural and Biological Engineering, 13(1), 247–254. Retrieved from https://ijabe.migration.pkpps03.publicknowledgeproject.org/index.php/ijabe/article/view/5128
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Renewable Energy and Material Systems
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