Effects of short light/dark cycles on photosynthetic pathway switching and growth of medicinal Dendrobium officinale in aeroponic cultivation
Keywords:
dark net CO2 exchange percentage, photosynthetic pathway, short light/dark cycle, soluble polysaccharidesAbstract
Dendrobium officinale has high medicinal value but grows slowly in natural environment due to its special CAM photosynthetic pathway. In this study, D. officinale were grown aeroponically with light/dark cycles of 12 h/12 h, 4 h/4 h, and 2 h/2 h for 150 d. The photosynthetic electron transfer characteristics, photosynthetic CO2 fixation pathways, and accumulations of biomass and soluble polysaccharides in D. officinale leaves were studied. The results showed that the photosynthetic apparatus states of D. officinale in aeroponic cultivation under short light/dark cycles of 4 h/4 h and 2 h/2 h were better than that under 12 h/12 h. The dark net CO2 exchange percentages of D. officinale were negative in short light/dark cycles of 4 h/4 h and 2 h/2 h, and the daily net CO2 exchange amount and dry/fresh weight increases were doubled compared with those in 12 h/12 h light/dark cycle. High soluble polysaccharides content and the soluble polysaccharides yield of D. officinale were obtained in the shorter light/dark cycle of 2 h/2 h. Therefore, the photosynthetic pathway of D. officinale could be switched from CAM to C3 by short light/dark cycles of 4 h/4 h and 2 h/2 h, and its higher biomass accumulation and soluble polysaccharides yield could be obtained by the shorter light/dark cycle of 2 h/2 h in aeroponic cultivation. Keywords: dark net CO2 exchange percentage, photosynthetic pathway, short light/dark cycle, soluble polysaccharides DOI: 10.25165/j.ijabe.20191205.4864 Citation: Cheng Y S, He D X, He J, Niu G H, Ji F. Effects of short light/dark cycles on photosynthetic pathway switching and growth of medicinal Dendrobium officinale in aeroponic cultivation. Int J Agric & Biol Eng, 2019; 12(5): 38–43.References
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[28] Lin X, Lai Z. Effects of light quality on expression of PEPC and polysaccharide accumulation in Dendrobium officinale. Chinese Journal of Tropical Crops, 2017; 5: 61–65. (in Chinese)
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[30] Nimmo H G. The regulation of phosphoenolpyruvate carboxylase in CAM plants. Trends in Plant Science, 2000; 5(2): 75–80.
[2] Borland A M, Griffiths H, Broadmeadow M S J, Fordham M C, Maxwell C. Short-term changes in carbon-isotope discrimination in the C3-CAM intermediate Clusia minor L. growing in Trinidad. Oecologia, 1993; 95(3): 444–453.
[3] Osmond C B. Crassulacean acid metabolism - curiosity in context. Annual Review of Plant Physiology and Plant Molecular Biology, 1978; 29: 379–414.
[4] Cockburn W, Ting I P. Relationships between stomatal behavior and internal carbon dioxide concentration in crassulacean acid metabolism plants. Plant Physiology, 1979; 63(6): 1029–1032.
[5] Winter K, Smith J A C. Crassulacean acid metabolism: biochemistry, ecophysiology, and evolution. Berlin: Springer-Verlag, 1996; pp.406–416.
[6] Gregory F G, Thimann K V. The interrelation between CO2 metabolism and photoperiodism in Kalanchoë. Plant Physiology, 1954; 29: 414.
[7] Queiroz O, Morel C. Photoperiodism and enzyme activity. Plant Physiolology, 1974; 4: 596–602.
[8] Wilkins M B. An endogenous rhythm in the rate of carbon dioxide output of Bryophyllum. IV. Effect of intensity of illumination on entrainment of the rhythm by cycles of light & darkness. Plant Physiology, 1962; 37: 735–741.
[9] Sekizuka F, Nose A, Kavamitsu Y, Murayama S, Arisumi K I. Effects of day length on gas exchange characteristics in the crassulacean acid metabolism plant Dendrobium ekapol cv. Panda. Japanese Journal of Crop Science, 1995; 64: 201–208.
[10] Kim H J, Ju H L, Lee H B, An S K, Kim K S. CO2 uptake behavior and vegetative growth of Doritaenopsis Queen Beer ‘Mantefon’ orchids as influenced by light/dark cycle manipulation. Flower Research Journal, 2017; 25: 253–261.
[11] Zhang Z, He D, Niu G, Gao R. Concomitant CAM and C3 photosynthetic pathways in Dendrobium officinale plants. Journal of the American Society for Horticultural Science, 2014; 139(3): 290–298.
[12] Cheng Y, He D, He J, Niu G, Gao R. Effect of light/dark cycle on photosynthetic pathway switching and CO2 absorption in two Dendrobium species. Frontiers in Plant Science, 2019; 10: 659.
[13] He J, Lee S K. Growth and photosynthetic responses of three aeroponically grown lettuce cultivars (Lactuca sativa L.) to different rootzone temperatures and growth irradiances under tropical aerial conditions. Journal of Pomology & Horticultural Science, 2015; 73(2): 173–180.
[14] Biddinger E J, Liu C, Joly R J, Raghothama K G. Physiological and molecular responses of aeroponically grown tomato plants to phosphorus deficiency. Journal of the American Society for Horticultural Science, 1998; 123(2): 330–333.
[15] Molitor H D, Fischer M, Popadopoulos A P. Effect of several parameters on the growth of chrysanthemum stock plants in aeroponics. Acta Horticulturae, 1999; 481: 179–186.
[16] Scoggins H L, Mills H A. Poinsettia growth, tissue nutrient concentration, and nutrient uptake as influenced by nitrogen form and stage of growth. Journal of Plant Nutrition, 1998; 21(1): 191–198.
[17] Dai G. Aeroponic cultivation method of Dendrobium officinale. Chinese patent, CN102972269A, 2013-03-20. (in Chinese)
[18] Huang Q. A method of aeroponic cultivation of Dendrobium officinale. Chinese Patent, CN106376445A, 2017-02-08. (in Chinese)
[19] Yang J, Chen C, Han X, Li X, Liebig H P. Measurement of vegetable leaf area using digital image processing techniques. Transactions of the CSAE, 2002; 18(4): 155–158
[20] Li M, Xu G, Hirata Y, Niwa M. Quantitative analysis of polysaccharides in Chinese Grug “Shihu” (Dendrobium). Chinese Herbal Medicines, 1990; 10: 10–12.
[21] Bjorkman D, Demmnig B. Comparison of the effect of excessive light on chlorophyll florescence (77K) and photon yield of O2 evolution in leaves of higher-plants. Planta, 1987; 171(2): 171–184.
[22] Appenroth K J, Stöckel J, Srivastava A, Strasser R J. Multiple effects of chromate on the photosynthetic apparatus of Spirodela polyrhiza as probed by OJIP chlorophyll a fluorescence measurements. Environmental Pollution, 2001; 115(1): 49–64.
[23] Van Heerden P D R, Strasser R J, Krüger G H J. Reduction of dark chilling stress in N2-fixing soybean by nitrate as indicated by chlorophyll a fluorescence kinetics. Physiologia Plantarum, 2004; 121: 239–249.
[24] Terashima I, Hanba Y T, Tholen D, Niinemets U. Leaf functional anatomy in relation to photosynthesis. Plant Physiology, 2001; 155: 108–116.
[25] Mancinelli A L, Rossi F, Moroni A. Chryptochrome, phytochrome, and anthocyanin production. Plant Physiology, 1991; 96: 1079–1085.
[26] Kurata H, Mochizuki A, Okuda N, Seki M, Furusaki S. Intermittent light irradiation with second- or hour-scale periods controls anthocyanin production by strawberry cells. Enzyme and Microbial Technology, 2000; 26: 621–629.
[27] Xu B, Cui Y, Guo C, Xia G. Dynamic variation of biomass and content of polysaccharide and alkaloid in protocorm like bodies from Dendrobium officinale at different light intensities and incubation time. Chinese Traditional and Herbal Drugs, 2012; 43(2): 355–359. (in Chinese)
[28] Lin X, Lai Z. Effects of light quality on expression of PEPC and polysaccharide accumulation in Dendrobium officinale. Chinese Journal of Tropical Crops, 2017; 5: 61–65. (in Chinese)
[29] Rolland F, Baena-Gonzalez E, Sheen J. Sugar sensing and signaling in plants: conserved and novel mechanisms. Annual Review of Plant Biology, 2006; 57: 675–709.
[30] Nimmo H G. The regulation of phosphoenolpyruvate carboxylase in CAM plants. Trends in Plant Science, 2000; 5(2): 75–80.
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2019-10-14
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Cheng, Y., He, D., He, J., Niu, G., & Ji, F. (2019). Effects of short light/dark cycles on photosynthetic pathway switching and growth of medicinal Dendrobium officinale in aeroponic cultivation. International Journal of Agricultural and Biological Engineering, 12(5), 38–43. Retrieved from https://ijabe.migration.pkpps03.publicknowledgeproject.org/index.php/ijabe/article/view/4864
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Animal, Plant and Facility Systems
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