Improving biomethane yield by strengthening acidification of maize stover in two-phase anaerobic digestion
Keywords:
alkaline pretreatment, two-phase anaerobic digestion, strengthening acidification, maize stover, reactor, biogas, biomethane productionAbstract
In this study, the acidification and two-phase anaerobic digestion (AD) were conducted in batch and continuous stirred tank reactors, respectively, to determine the effect of acidification on methane production in AD. The results showed that two-phase AD achieved an observable enhancement in the methane production under optimal acidification conditions (organic loading rate of 60 g TS/L, the ratio of raw material to inoculum (based on dry weight) of 2:1, the temperature of 45°C, urea concentration of 4%, and time of 6 d). Under these conditions, the daily biogas and biomethane productions were 0.48 L/g TS and 0.30 L/g TS, respectively, which were 26.32% and 57.89% higher than those of the untreated group, respectively. The ammonia nitrogen (AN), alkalinity, and pH value of the methanogenic phase of C4 continued to increase up to 956 mg/L, 5680 mg/L, and 7.41, respectively, after 60 d, which might have destroyed the stability of the system. Therefore, for the purpose of reusing the nitrogen source, reducing AN, and maintaining the stability of the reaction system, another set of acidification and two-phase AD with water pretreatment using the discharge of the methanogenic phase of C4 as the inoculum was subsequently conducted. The results showed that the daily biogas productions of single-phase and two-phase AD were 5.26% and 15.79% higher than that of the untreated group, respectively; similarly, their daily methane yields were 10.42% and 21.05% higher than that of the untreated group. Keywords: alkaline pretreatment, two-phase anaerobic digestion, strengthening acidification, maize stover, reactor, biogas, biomethane production DOI: 10.25165/j.ijabe.20201304.4654 Citation: Tong H, Zhou B Y, Liu C M, Wachemo A C, Li X J, Zuo X Y. Improving biomethane yield by strengthening acidification of maize stover in two-phase anaerobic digestion. Int J Agric & Biol Eng, 2020; 13(4): 226–231.References
[1] National Bureasu of Statistics of the People’s Republic of China, 2016. http://www.stats.gov.cn/.
[2] Zheng M X, Li X J, Li L Q, Yang X J, He Y F. Enhancing anaerobic biogasification of corn stover through wet state NaOH pretreatment. Bioresource Technology, 2009; 100(21): 5140–5145.
[3] Wang G, Gavala H N, Skiadas I V, Ahring B K. Wet explosion of wheat straw and codigestion with swine manure: Effect on the methane productivity. Waste Manag, 2009; 29(11): 2830–2835.
[4] Wang F, Yin H, Li S. China’s renewable energy policy: Commitments and challenges. Energy Policy, 2010; 38(4): 1872–1878.
[5] Li Y Q, Liu C M, Wachemo A C, Yuan H R, Zou D X, Liu Y P, et al. Serial completely stirred tank reactors for improving biogas production and substance degradation during anaerobic digestion of corn stover. Bioresour Technol., 2017; 235: 380–388.
[6] Tsavkelova E A. Biogas production from cellulose-containing substrates: A review. Applied Biochemistry & Microbiology, 2012; 48(5): 421–433.
[7] Deswarte F E I, Clark J H, Wilson A J, Hardy J J E, Marriott R, Chahal S P, et al. Toward an integrated straw-based biorefinery. Biofuels Bioproducts & Biorefining, 2010; 1(4): 245–254.
[8] Smith D P, Mccarty P L. Reduced product formation following perturbation of ethanol- and propionate-fed methanogenic CSTRs. Biotechnology & Bioengineering, 1989; 34(7): 885–895.
[9] Bouallagui H, Torrijos M, Godon J J, Moletta R, Cheikh R B, Touhami Y, et al. Two-phases anaerobic digestion of fruit and vegetable wastes: Bioreactors performance. Biochemical Engineering Journal, 2004; 21(2): 193–197.
[10] Cysneiros D, Banks C J, Heaven S, Karatzas K A. The role of phase separation and feed cycle length in leach beds coupled to methanogenic reactors for digestion of a solid substrate (Part 2): Hydrolysis, acidification and methanogenesis in a two-phase system. Bioresource Technology, 2011; 102(16): 7393–7400.
[11] Grover R, Marwaha S S, Kennedy J F. Methanogenesis of black liquor in a two-stage biphasic reactor system using an immobilized cell system. Journal of Chemical Technology & Biotechnology, 2010; 76(3): 251–256.
[12] Roberts R, Davies W J, Forster C F. Two-stage, thermophilic-mesophilic anaerobic digestion of sewage sludge. Process Safety & Environmental Protection, 1999; 77(2): 93–97.
[13] Grimberg S J, Hilderbrandt D, Kinnunen M, Rogers S. Anaerobic digestion of food waste through the operation of a mesophilic two-phase pilot scale digester - Assessment of variable loadings on system performance. Bioresource Technology, 2015; 178: 226–229.
[14] Demi̇Rer G N, Chen S. Two-phase anaerobic digestion of unscreened dairy manure. Process Biochemistry, 2005; 40(11): 3542–3549.
[15] Massanetnicolau J, Dinsdale R, Guwy A, Shipley G. Use of real time gas production data for more accurate comparison of continuous single-stage and two-stage fermentation. Bioresource Technology, 2013; 129(2): 561–567.
[16] Min L, Ren N Q, Ying C, Zhu W F, Jie D. Conversion regular patterns of acetic acid, propionic acid and butyric acid in UASB reactor. Journal of Enviermental Sciences, 2004; 16(3): 387–391.
[17] Lay J J. Modeling and optimization of anaerobic digested sludge converting starch to hydrogen. Biotechnology & Bioengineering, 2015; 68(3): 269–278.
[18] Cha G C, Noike T. Effect of rapid temperature change and HRT on anaerobic acidogenesis. Water Science & Technology, 1997; 36(6-7): 247–253.
[19] Chen H, Meng H, Nie Z, Zhang M. Polyhydroxyalkanoate production from fermented volatile fatty acids: effect of pH and feeding regimes. Bioresour Technol., 2013; 128(1): 533–538.
[20] Jiang J, Zhang Y, Li K, Wang Q, Gong C, Li M. Volatile fatty acids production from food waste: Effects of pH, temperature, and organic loading rate. Bioresour Technol., 2013; 143(9): 525–530.
[21] Liu X, Dong B, Dai X. Hydrolysis and acidification of dewatered sludge under mesophilic, thermophilic and extreme thermophilic conditions: Effect of pH. Bioresource Technology, 2013; 148(8): 461–466.
[22] Zhou A, Guo Z, Yang C, Kong F, Liu W, Wang A. Volatile fatty acids productivity by anaerobic co-digesting waste activated sludge and corn straw: Effect of feedstock proportion. Journal of Biotechnology, 2013; 168(2): 234–239.
[23] Rajagopal R, Massé D I, Singh G. A critical review on inhibition of anaerobic digestion process by excess ammonia. Bioresource Technology, 2013; 143(17): 632–641.
[24] Liu C F, Yuan X Z, Zeng G M, Li W W, Li J. Prediction of methane yield at optimum pH for anaerobic digestion of organic fraction of municipal solid waste. Bioresour Technol., 2008; 99(4): 882–888.
[2] Zheng M X, Li X J, Li L Q, Yang X J, He Y F. Enhancing anaerobic biogasification of corn stover through wet state NaOH pretreatment. Bioresource Technology, 2009; 100(21): 5140–5145.
[3] Wang G, Gavala H N, Skiadas I V, Ahring B K. Wet explosion of wheat straw and codigestion with swine manure: Effect on the methane productivity. Waste Manag, 2009; 29(11): 2830–2835.
[4] Wang F, Yin H, Li S. China’s renewable energy policy: Commitments and challenges. Energy Policy, 2010; 38(4): 1872–1878.
[5] Li Y Q, Liu C M, Wachemo A C, Yuan H R, Zou D X, Liu Y P, et al. Serial completely stirred tank reactors for improving biogas production and substance degradation during anaerobic digestion of corn stover. Bioresour Technol., 2017; 235: 380–388.
[6] Tsavkelova E A. Biogas production from cellulose-containing substrates: A review. Applied Biochemistry & Microbiology, 2012; 48(5): 421–433.
[7] Deswarte F E I, Clark J H, Wilson A J, Hardy J J E, Marriott R, Chahal S P, et al. Toward an integrated straw-based biorefinery. Biofuels Bioproducts & Biorefining, 2010; 1(4): 245–254.
[8] Smith D P, Mccarty P L. Reduced product formation following perturbation of ethanol- and propionate-fed methanogenic CSTRs. Biotechnology & Bioengineering, 1989; 34(7): 885–895.
[9] Bouallagui H, Torrijos M, Godon J J, Moletta R, Cheikh R B, Touhami Y, et al. Two-phases anaerobic digestion of fruit and vegetable wastes: Bioreactors performance. Biochemical Engineering Journal, 2004; 21(2): 193–197.
[10] Cysneiros D, Banks C J, Heaven S, Karatzas K A. The role of phase separation and feed cycle length in leach beds coupled to methanogenic reactors for digestion of a solid substrate (Part 2): Hydrolysis, acidification and methanogenesis in a two-phase system. Bioresource Technology, 2011; 102(16): 7393–7400.
[11] Grover R, Marwaha S S, Kennedy J F. Methanogenesis of black liquor in a two-stage biphasic reactor system using an immobilized cell system. Journal of Chemical Technology & Biotechnology, 2010; 76(3): 251–256.
[12] Roberts R, Davies W J, Forster C F. Two-stage, thermophilic-mesophilic anaerobic digestion of sewage sludge. Process Safety & Environmental Protection, 1999; 77(2): 93–97.
[13] Grimberg S J, Hilderbrandt D, Kinnunen M, Rogers S. Anaerobic digestion of food waste through the operation of a mesophilic two-phase pilot scale digester - Assessment of variable loadings on system performance. Bioresource Technology, 2015; 178: 226–229.
[14] Demi̇Rer G N, Chen S. Two-phase anaerobic digestion of unscreened dairy manure. Process Biochemistry, 2005; 40(11): 3542–3549.
[15] Massanetnicolau J, Dinsdale R, Guwy A, Shipley G. Use of real time gas production data for more accurate comparison of continuous single-stage and two-stage fermentation. Bioresource Technology, 2013; 129(2): 561–567.
[16] Min L, Ren N Q, Ying C, Zhu W F, Jie D. Conversion regular patterns of acetic acid, propionic acid and butyric acid in UASB reactor. Journal of Enviermental Sciences, 2004; 16(3): 387–391.
[17] Lay J J. Modeling and optimization of anaerobic digested sludge converting starch to hydrogen. Biotechnology & Bioengineering, 2015; 68(3): 269–278.
[18] Cha G C, Noike T. Effect of rapid temperature change and HRT on anaerobic acidogenesis. Water Science & Technology, 1997; 36(6-7): 247–253.
[19] Chen H, Meng H, Nie Z, Zhang M. Polyhydroxyalkanoate production from fermented volatile fatty acids: effect of pH and feeding regimes. Bioresour Technol., 2013; 128(1): 533–538.
[20] Jiang J, Zhang Y, Li K, Wang Q, Gong C, Li M. Volatile fatty acids production from food waste: Effects of pH, temperature, and organic loading rate. Bioresour Technol., 2013; 143(9): 525–530.
[21] Liu X, Dong B, Dai X. Hydrolysis and acidification of dewatered sludge under mesophilic, thermophilic and extreme thermophilic conditions: Effect of pH. Bioresource Technology, 2013; 148(8): 461–466.
[22] Zhou A, Guo Z, Yang C, Kong F, Liu W, Wang A. Volatile fatty acids productivity by anaerobic co-digesting waste activated sludge and corn straw: Effect of feedstock proportion. Journal of Biotechnology, 2013; 168(2): 234–239.
[23] Rajagopal R, Massé D I, Singh G. A critical review on inhibition of anaerobic digestion process by excess ammonia. Bioresource Technology, 2013; 143(17): 632–641.
[24] Liu C F, Yuan X Z, Zeng G M, Li W W, Li J. Prediction of methane yield at optimum pH for anaerobic digestion of organic fraction of municipal solid waste. Bioresour Technol., 2008; 99(4): 882–888.
Downloads
Published
2020-08-07
How to Cite
Tong, H., Zhou, B., Liu, C., Wachemo, A. C., Li, X., & Zuo, X. (2020). Improving biomethane yield by strengthening acidification of maize stover in two-phase anaerobic digestion. International Journal of Agricultural and Biological Engineering, 13(4), 226–231. Retrieved from https://ijabe.migration.pkpps03.publicknowledgeproject.org/index.php/ijabe/article/view/4654
Issue
Section
Renewable Energy and Material Systems
License
IJABE is an international peer reviewed open access journal, adopting Creative Commons Copyright Notices as follows.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
