Microorganism population in two-phase anaerobic fermentation of separated liquid of dairy manure
Keywords:
separated liquid, diluted dairy manure, two-phase anaerobic fermentation, microorganism, scanning electron microscope (SEM), Denaturing Gradient Gel Electrophoresis (DGGE)Abstract
In order to reduce incomplete fermentation caused by high substrate viscosity and low mass transfer efficiency during fermentation process, batch and two-phase anaerobic fermentation experiments were conducted in this study. Dairy manure was separated by using solid-liquid separator firstly. Separated liquid (SL) and diluted dairy manure (DDM) as raw materials were evaluated in terms of gas production performance for both batch and two-phase anaerobic fermentation. The microorganism population was characterized by scanning electron microscope (SEM) and Denaturing Gradient Gel Electrophoresis (DGGE). The results showed that Volatile Solid (VS) methane yield of SL was 124.51 L/kg VS, which was 2.09 times higher than that of DDM (59.50 L/kg VS) in batch anaerobic fermentation. The Bacteroides and Veillonella with higher activity were the majority microorganism population in acidogenic phase, whereas the Firmicutes and Corynebacterium with methanogenic properties became the predominant microorganism population in methanogenic phase. This study achieved the phase separation and improved the gas production performance. Keywords: separated liquid, diluted dairy manure, two-phase anaerobic fermentation, microorganism, scanning electron microscope (SEM), Denaturing Gradient Gel Electrophoresis (DGGE) DOI: 10.25165/j.ijabe.20181101.3206 Citation: Li Q, Guan Z J, Zheng G X. Microorganism population in two-phase anaerobic fermentation of separated liquid of dairy manure. Int J Agric & Biol Eng, 2018; 11(1): 206–211.References
[1] Liu G Q, Dong R J, Li X Q. Biomass utilization technologies. Beijing: Chemical Industry Press, 2009. (in Chinese)
[2] Steinfeld H, Costales A, Rushton J, Scherf B, Bennett T, Hall D. Livestock Report, FAO, Rome. 2006.
[3] Cambra-López M, Aarnink A J, Zhao Y, Calvet S, Torres A G. Airborne particulate matter from livestock production systems: A review of an air pollution problem. EnvironmentPollution, 2010; 158(1): 1–17.
[4] Petersen S O, Sommer S G, Béline F, Burton C, Dach J, Dourmad J Y. Recycling of livestock manure in a whole-farm perspective. Livestock Science, 2007; 112(3): 180–191.
[5] Amann R I, Ludwig W, Schleifer K H. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiological reviews, 1995; 59(1): 143–169.
[6] Muyzer G, de Waal E C, Uitterlinden A G. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Applied and environmental microbiology, 1993; 59(3): 695–700.
[7] Aydin S, Shahi A, Ozbayram G, Ince B, Ince O. Use of PCR-DGGE based molecular methods to assessment of microbial diversity during anaerobic treatment of antibiotic combinations. Bioresource Technology, 2015; 192: 735–740.
[8] Muyzer G, Waalec D, Uitterlinden G. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol, 1993; 59: 695–700.
[9] Delgado S, Arroyo R, Martín R, Martín R, Rodríguez J M. PCR-DGGE assessment of the bacterial diversity of breast milk in women with
lactational infectious mastitis. BMC infectious diseases, 2008; 8(1): 51.
[10] Shi X S, Liu D L, Lang Z H, Huang D F, Lu W, Li M. A preliminary application of PCR-DGGE in microbial community structure analysis of rural household biogas digesters. Journal of Shandong Normal University (Natural Science), 2007; 22(2): 120–122. (in Chinese)
[11] Wang Y W, Xu F H, Yuan Z Y, Song J L, Wang Q, Zhao B. Study on the methanogenic archaea communities in low temperature biogas digester by DGGE and Real-Time PCR methods. China Biogas, 2012; 30(1): 8–12. (in Chinese)
[12] Gao X X, Zhao L X, Dong B C, Wan X C, Chen L, Luo J. Experimental study on effect of separated two-phase anaerobic fermentation leachate recirculation on anaerobic process. Transactions of the CSAE, 2011; 27(10): 266–269. ( in Chinese)
[13] Dong B C, Chen L, Zhang Y H, Zhao L X, Wan X C, Gao X X. Effect of pig manure addition on biogas production of combined two-phase anaerobic digestion. Transctions of the CSAE, 2011; 27(Supp.1): 48–52. (in Chinese)
[14] Guan Z J, Li W Z, Zheng G X, Bi L P. Effect of solid-liquid separation on utilization of dairy manure. Transactions of the CSAE, 2011; 27(4): 259–263. (in Chinese)
[15] El-Mashad H M, Zhang R. Biogas production from co-digestion of dairy manure and food waste. Bioresource Technology, 2010; 101(11): 4021-4028.
[16] Marañón E, Castrillón L, Quiroga G, Fernández-Nava Y, Gómez L, García M M. Co-digestion of cattle manure with food waste and sludge to increase biogas production. Waste management, 2012; 32(10): 1821–1825.
[17] Amon T, Amon B, Kryvoruchko V, Zollitsch W, Mayer K, Gruber L. Biogas production from maize and dairy cattle manure-influence of biomass composition on the methane yield. Agriculture, Ecosystems & Environment, 2007; 118(1): 173-182.
[18] Yue Z, Chen R, Yang F, James MacLellan, Terence Marsh, Liu Y. Effects of dairy manure and corn stover co-digestion on anaerobic microbes and corresponding digestion performance. Bioresource Technology, 2013; 128: 65–71.
[19] Demirer G N, Chen S. Two-phase anaerobic digestion of unscreened dairy manure. Process Biochemistry, 2005; 40(11): 3542–3549.
[20] Guan Z J, Li W Z, Zheng G X, Bi L P. Technology for two-phase anaerobic fermentation by solid-liquid separated solution of dairy manure. Transactions of the CSAE, 2011; 27(7): 300–305. (in Chinese)
[21] Federation W E. Standard methods for the examination of water and wastewater. American Public Health Association, American Water Works Association, and Water Environment Federation, 2005.
[22] Gao P P, Zhao L P. DNA extraction from activated sludge for molecular community analysis. Acta Ecologica Sinica, 2002; 22(11): 2015–2019.
[23] Thompson J R, Marcelino L A, Polz M F. Heteroduplexes in mixed-template amplifications: formation, consequence and elimination by ‘reconditioning PCR’. Nucleic Acids Research, 2002; 30(9): 2083–2088.
[24] Wintzingerode F, Göbel U B, Stackebrandt E. Determination of microbial diversity in environmental samples: Pitfalls of PCR-based rRNA analysis. FEMS Microbiology Reviews, 1997; 21(3): 213–229.
[25] Xing W, Zuo J E, Sun Y J, Li J P. Study on microbial community in methanogenic granular sludge by FISH and DGGE. Environmental Science, 2006; 27(11): 2268–2272.
[2] Steinfeld H, Costales A, Rushton J, Scherf B, Bennett T, Hall D. Livestock Report, FAO, Rome. 2006.
[3] Cambra-López M, Aarnink A J, Zhao Y, Calvet S, Torres A G. Airborne particulate matter from livestock production systems: A review of an air pollution problem. EnvironmentPollution, 2010; 158(1): 1–17.
[4] Petersen S O, Sommer S G, Béline F, Burton C, Dach J, Dourmad J Y. Recycling of livestock manure in a whole-farm perspective. Livestock Science, 2007; 112(3): 180–191.
[5] Amann R I, Ludwig W, Schleifer K H. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiological reviews, 1995; 59(1): 143–169.
[6] Muyzer G, de Waal E C, Uitterlinden A G. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Applied and environmental microbiology, 1993; 59(3): 695–700.
[7] Aydin S, Shahi A, Ozbayram G, Ince B, Ince O. Use of PCR-DGGE based molecular methods to assessment of microbial diversity during anaerobic treatment of antibiotic combinations. Bioresource Technology, 2015; 192: 735–740.
[8] Muyzer G, Waalec D, Uitterlinden G. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol, 1993; 59: 695–700.
[9] Delgado S, Arroyo R, Martín R, Martín R, Rodríguez J M. PCR-DGGE assessment of the bacterial diversity of breast milk in women with
lactational infectious mastitis. BMC infectious diseases, 2008; 8(1): 51.
[10] Shi X S, Liu D L, Lang Z H, Huang D F, Lu W, Li M. A preliminary application of PCR-DGGE in microbial community structure analysis of rural household biogas digesters. Journal of Shandong Normal University (Natural Science), 2007; 22(2): 120–122. (in Chinese)
[11] Wang Y W, Xu F H, Yuan Z Y, Song J L, Wang Q, Zhao B. Study on the methanogenic archaea communities in low temperature biogas digester by DGGE and Real-Time PCR methods. China Biogas, 2012; 30(1): 8–12. (in Chinese)
[12] Gao X X, Zhao L X, Dong B C, Wan X C, Chen L, Luo J. Experimental study on effect of separated two-phase anaerobic fermentation leachate recirculation on anaerobic process. Transactions of the CSAE, 2011; 27(10): 266–269. ( in Chinese)
[13] Dong B C, Chen L, Zhang Y H, Zhao L X, Wan X C, Gao X X. Effect of pig manure addition on biogas production of combined two-phase anaerobic digestion. Transctions of the CSAE, 2011; 27(Supp.1): 48–52. (in Chinese)
[14] Guan Z J, Li W Z, Zheng G X, Bi L P. Effect of solid-liquid separation on utilization of dairy manure. Transactions of the CSAE, 2011; 27(4): 259–263. (in Chinese)
[15] El-Mashad H M, Zhang R. Biogas production from co-digestion of dairy manure and food waste. Bioresource Technology, 2010; 101(11): 4021-4028.
[16] Marañón E, Castrillón L, Quiroga G, Fernández-Nava Y, Gómez L, García M M. Co-digestion of cattle manure with food waste and sludge to increase biogas production. Waste management, 2012; 32(10): 1821–1825.
[17] Amon T, Amon B, Kryvoruchko V, Zollitsch W, Mayer K, Gruber L. Biogas production from maize and dairy cattle manure-influence of biomass composition on the methane yield. Agriculture, Ecosystems & Environment, 2007; 118(1): 173-182.
[18] Yue Z, Chen R, Yang F, James MacLellan, Terence Marsh, Liu Y. Effects of dairy manure and corn stover co-digestion on anaerobic microbes and corresponding digestion performance. Bioresource Technology, 2013; 128: 65–71.
[19] Demirer G N, Chen S. Two-phase anaerobic digestion of unscreened dairy manure. Process Biochemistry, 2005; 40(11): 3542–3549.
[20] Guan Z J, Li W Z, Zheng G X, Bi L P. Technology for two-phase anaerobic fermentation by solid-liquid separated solution of dairy manure. Transactions of the CSAE, 2011; 27(7): 300–305. (in Chinese)
[21] Federation W E. Standard methods for the examination of water and wastewater. American Public Health Association, American Water Works Association, and Water Environment Federation, 2005.
[22] Gao P P, Zhao L P. DNA extraction from activated sludge for molecular community analysis. Acta Ecologica Sinica, 2002; 22(11): 2015–2019.
[23] Thompson J R, Marcelino L A, Polz M F. Heteroduplexes in mixed-template amplifications: formation, consequence and elimination by ‘reconditioning PCR’. Nucleic Acids Research, 2002; 30(9): 2083–2088.
[24] Wintzingerode F, Göbel U B, Stackebrandt E. Determination of microbial diversity in environmental samples: Pitfalls of PCR-based rRNA analysis. FEMS Microbiology Reviews, 1997; 21(3): 213–229.
[25] Xing W, Zuo J E, Sun Y J, Li J P. Study on microbial community in methanogenic granular sludge by FISH and DGGE. Environmental Science, 2006; 27(11): 2268–2272.
Downloads
Published
2018-01-31
How to Cite
Li, Q., Guan, Z., & Zheng, G. (2018). Microorganism population in two-phase anaerobic fermentation of separated liquid of dairy manure. International Journal of Agricultural and Biological Engineering, 11(1), 206–211. Retrieved from https://ijabe.migration.pkpps03.publicknowledgeproject.org/index.php/ijabe/article/view/3206
Issue
Section
Biosystems, Biological and Ecological Engineering
License
IJABE is an international peer reviewed open access journal, adopting Creative Commons Copyright Notices as follows.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
