Early diagnosis and monitoring of nitrogen nutrition stress in tomato leaves using electrical impedance spectroscopy
Keywords:
electrical impedance spectroscopy, nitrogen stress, tomato (Solanum lycopersicum) leaves, nitrogen nutrition, diagnosis, monitoring, nondestructive detectionAbstract
Abstract: Nitrogen (N) is a life element for crop growth. In tomato growth and development, N stress often occurs and degrades crop yield and quality. Superfluous N can noticeably increase the nitrate content, which can be degraded into strong carcinogenic substance- nitrite. An accurate and timely monitoring and diagnosis of nutrition during crop growth is premise to realize a precise nutrient management. Crop N monitoring methods have been developed to improve N fertilizer management, and most of them are based on leaf or canopy optical property measurements. Although many optical/spectral plant N sensors have already commercialized for production use, low accuracy for phosphorus (P) and potassium (K) detection and diagnosis remains an important drawback of these methods. To explore the potential of N diagnosis by electrical impedance and perform study for nutrition status of plant NPK meanwhile by the electrical impedance, it is necessary that evaluate the N nutrition level by leaf impedance spectroscopy. Electrical impedance was applied to determine the physiological and nutritional status of plant tissues, but few studies related to plant N contents have been reported. The objective of this study was to evaluate the N nutrition level by leaf impedance spectroscopy and realize the early diagnosis and monitoring of N nutrition stress in tomato. Five sets of tomato plant samples with different N contents were cultivated in a Venlo greenhouse. N content of leaves was determined, and electrical impedance data were recorded in a frequency range of 1 Hz to 1 MHz. The obtained impedance data were analyzed using an equivalent circuit model for cellular tissues. The variation of equivalent parameters along with N content was analyzed, and the sensitive impedance spectroscopy characteristics of N nutrition level were extracted. Furthermore, the effect of moisture content on impedance measurement was discussed and the prediction model for N content was developed. Results showed that electrical impedance can be conveniently applied to early diagnosis and monitoring for tomato N nutrition stress. Keywords: electrical impedance spectroscopy, nitrogen stress, tomato (Solanum lycopersicum) leaves, nitrogen nutrition, diagnosis, monitoring, nondestructive detection DOI: 10.3965/j.ijabe.20171003.3188 Citation: Li M Q, Li J Y, Wei X H, Zhu W J. Early diagnosis and monitoring of nitrogen nutrition stress in tomato leaves using electrical impedance spectroscopy. Int J Agric & Biol Eng, 2017; 10(3): 194–205.References
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[2] Azzarello E, Masi E, Mancuso S. Electrochemical impedance spectroscopy: in plant electrophysiology. Germany: Heidelberg. 2012; pp. 205–223.
[3] Khaled D El, Castellano N N, Gazquez J A, García Salvador R M, Manzano-Agugliaro F. Cleaner quality control system using bioimpedance methods: a review for fruits and vegetables. Journal of Cleaner Production, 2015; 140: 1749–1762.
[4] Tomkiewicz D, Piskier T. A plant based sensing method for nutrition stress monitoring. Precis. Agric, 2012; 13(3): 370–383.
[5] Liu Y, Wang T, Wu H Y. Diagnosis on potassium nutrition of maize using impedance parameter of leaf Tissue Juice. Transactions of the CSAM, 2013; 44(1): 185–189. (in Chinese)
[6] Muñoz-Huerta R F, Ortiz-Melendez A D J, Guevara- Gonzalez R G, Torres-Pacheco I, Herrera-Ruiz G, Contreras-Medina L M, et al. An analysis of electrical impedance measurements applied for plant N status estimation in lettuce (Lactuca sativa). Sensors, 2014; 14: 11492–11503.
[7] Borges E, Matos A P, Cardoso J M. Early detection and monitoring of plant disease by bioelectric impedance spectroscopy. 2nd IEEE Portuguese Meeting, Bioengineering, 2012.
[8] Chowdhury A, Bera T K, Ghoshal D, Chakraborty B. Electrical impedance variations in banana ripening: an analytical study with electrical impedance spectroscopy. Journal of Food Process Engineering, 2017; 40(2): 1–14.
[9] Rehman M, Basem A J A, Izneid A, Abdullah M Z, Arshad M R. Assessment of quality of fruits using impedance spectroscopy. International Journal of Food Science and Technology, 2011; 46(6): 1303–1309.
[10] Freeborn T J, Maundy B, Elwakil A S. Cole impedance extractions from the step response of a current excited fruit sample. Computer and Electronics in Agriculture, 2013; 98: 100–108.
[11] Zhao P F, Zhang H L, Zhao D J, Wang Z J, Fan L F. Rapid on-line non-destructive detection of the moisture content of corn ear by bioelectrical impedance spectroscopy. Int J Agric & Biol Eng, 2015; 8(6): 37–45.
[12] Hamed K B, Zorrig W, Ahmed H H. Electrical impedance spectroscopy: A tool to investigate the responses of one halophyte to different growth and stress conditions. Computers and Electronics in Agriculture, 2016; 123: 376–383.
[13] Harry O L, Thierry B. Analysis of root growth by impedance spectroscopy (EIS). Plant and Soil, 2005; 277: 299–313.
[14] Ellis T, Murray W, Kavalieris L. Electrical capacitance of bean (Vicia faba) root systems was related to tissue density- a test for the Dalton Model. Plant Soil, 2013; 366: 575–584.
[15] Lu Y Z, Hu Y G, Zhang X L, Li P P. Responses of electrical properties of tea leaves to low-temperature stress. Int J Agric & Biol Eng, 2015; 8(5): 170–175.
[16] Zhang B H, Wang R H, Wang Y X, Li Y B. LabVIEW-based impedance biosensing system for detection of avian influenza virus. Int J Agric & Biol Eng, 2016; 9(4): 116–122.
[17] Swisher S L, Lin M C, Liao A, Leeflang E J, Khan Y. Impedance sensing device enables early detection of pressure ulcers in vivo. Nature Communications, 2015; 6: 65–75.
[18] Greenham C G, Randall P J, Müller W J. Studies of phosphorus and potassium deficiencies in Trifolium subterraneum based on electrical measurements. Can. J. Bot., 2011; 60(5): 634–644.
[19] Xiao J Z, Ren F L, Re S L T. Absorption rule of tomato on NPK and effect of fertilizer application. Xinjiang Agricultural Sciences, 1990; 3: 114–116. (in Chinese)
[20] Yu W G, Zhao T M. Tomato cultivation new technology. Fujian Science and Technology Press, 2005. (in Chinese)
[21] Bao S D. Soil agro-chemistrical analysis. China Agriculture Press, 2007. (in Chinese)
[22] Zoltowski P. On the electrical capacitance of interfaces exhibiting constantphase element behavior. Journal of Electroanalytical Chemistry, 1998; 443(1): 149–154.
[23] Larfaillou S, Guy-Bouyssou D, Cras F L, Franger S. Comprehensive characterization of all-solid-state thin films commercial microbatteries by Electrochemical Impedance Spectroscopy. Journal of Power Sources, 2016; 319: 139–146.
[24] Alexander C L, Tribollet B, Orazem M E. Contribution of surface distributions to constant-phase-element (CPE) behavior: 2 Capacitance. Electrochimica Acta, 2016; 188: 566–573.
[25] Córdoba-Torres P. Relationship between constant-phase element (CPE) parameters and physical properties of films with a distributed resistivity. Electrochimica Acta, 2017; 225: 592–604.
[26] Yasumasa A, Koichi M, Naoto W. Electrical impedance analysis of potato tissues during drying. Journal of Food Engineering, 2014; 121(1): 24–31.
[27] Li J Y, Li M Q, Mao H P, Zhu W J. Diagnosis of potassium nutrition level in Solanum lycopersicum based on electrical impedance. Biosystems Engineering, 2016; 147: 130–138.
[28] Mizukami Y, Sawai Y, Yamaguchi Y. Moisture content measurement of tea leaves by electrical impedance and capacitance. Biosystems Engineering, 2006; 93(3): 293–299.
[29] Li J Y, Mao H P. Monitoring of tomato leaf moisture content based on electrical impedance and capacitance. Transactions of the CSAM, 2016; 47(5): 293–298. (in Chinese)
[30] Takashima T, Hikosaka K, Hirose T. Photosynthesis or persistence: nitrogen allocation in leaves of evergreen and deciduous Quercus species. Plant, Cell & Environment, 2004; 27(8): 1047–1054.
[31] Shi Z M, Tang J C, Cheng R M, Luo D, Liu S R. A review of nitrogen allocation in leaves and factors in its effects. Acta Ecologica Sinica, 2015; 35(18): 5909–5919. (in Chinese)
[32] Masot R, Alcañiz M, Fuentes A, Schmidt F C, Barat J M, Gil L, et al. Design of a low-cost non-destructive system for punctual measurements of salt levels in food products using impedance spectroscopy. Sensors and Actuators A, 2010; 158(2): 217–223.
[33] Bezanilla F. White M M. Membrane transport processes in organized systems. Chapter I: Properties of ionic channels in excitable membranes. Plenum Medical Book Company, New York, US, 1987.
[34] Sun S W, Gao X Y, Lu Z G. Effects of different nitrogen fertilization levels on quality of tomato cultivated in solar greenhouse. Northern Horticulture, 2011; 11: 36–37. (in Chinese)
[35] Cseresnyés I, Rajkai K, Vozáry E.. Role of phase angle measurement in electrical impedance spectroscopy. Int. Agrophys, 2013; 27(4): 377–383.
[36] McAdams E T, Jossinet J. Problems in equivalent circuit modelling of the electrical properties of biological tissues. Bioelectrochemistry & Bioenergetics, 1996; 40(2): 147–152.
[37] Zhang M I N, Willison J H M. Electrical impedance analysis in plant tissues: a double shell model. J. Exp. Bot., 1991; 42(244): 1465–1475.
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Published
2017-05-31
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Meiqing, L., Jinyang, L., Xinhua, W., & Wenjing, Z. (2017). Early diagnosis and monitoring of nitrogen nutrition stress in tomato leaves using electrical impedance spectroscopy. International Journal of Agricultural and Biological Engineering, 10(3), 194–205. Retrieved from https://ijabe.migration.pkpps03.publicknowledgeproject.org/index.php/ijabe/article/view/3188
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Biosystems, Biological and Ecological Engineering
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