Improvement of microalgae lipid productivity and quality in an ion-exchange-membrane photobioreactor using real municipal wastewater
Keywords:
microalgae, photobioreactor, lipid productivity, real municipal wastewater, ion-exchange-membraneAbstract
To improve the productivity and quality of microalgae-based biodiesel when using municipal wastewater (MW) as nutrients source, an ion-exchange-membrane photobioreactor (IEM-PBR) was used in this study to eliminate the negative effects of pollutants in MW on microalgae Chlorella vulgaris and Scenedesmus obliquus. In the IEM-PBR, the real MW and microalgae cultures were separated in two chambers by the ion-exchange-membranes (IEMs). Nutrients (N, P, etc.) in the MW permeated into microalgae cultures through the IEMs, while pollutants (suspended solids, competitors, etc.) in the MW could hardly permeate into microalgae cultures. As a result, the lipid productivity in the IEM-PBR was improved to 85.7 mg/(L•d) for C. vulgaris and 111.8 mg/(L•d) for S. obliquus, which was slightly higher than that in the traditional photobioreactor (T-PBR) with real MW after centrifugation (82.5 mg/(L•d) for C. vulgaris and 105.8 mg/(L•d) for S. obliquus), but much higher than that in the T-PBR with untreated MW and primary MW (with lipid productivity of 20-30 mg/(L•d)). Besides, the lipid quality obtained in the IEM-PBR had higher proportion of cetane number (ca. 60%) and lower linolenic acid content (ca. 8%), which showed a superior quality in the IEM-PBR to that in the T-PBR. It demonstrated that the IEM-PBR is an effective approach to improve the productivity and quality of microalgae biodiesel. Keywords: microalgae, photobioreactor, lipid productivity, real municipal wastewater, ion-exchange-membrane DOI: 10.3965/j.ijabe.20171001.2706 Citation: Chang H X, Fu Q, Huang Y, Xia A, Liao Q, Zhu X. Improvement of microalgae lipid productivity and quality in an ion-exchange-membrane photobioreactor using real municipal wastewater. Int J Agric & Biol Eng, 2017; 10(1): 97-106.References
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[9] Ebrahimian A, Kariminia HR, Vosoughi M. Lipid production in mixotrophic cultivation of Chlorella vulgaris in a mixture of primary and secondary municipal wastewater. Renewable Energy, 2014; 71: 502–508.
[10] Chang H X, Fu Q, Huang Y, Xia A, Liao Q, Zhu X, et al. An annular photobioreactor with ion-exchange-membrane for non-touch microalgae cultivation with wastewater. Bioresource Technology, 2016; 219: 668–676.
[11] Bligh E G, Dyer W J. A rapid method of total lipid extraction and purification. Canadian Journal Biochemistry Physiology, 1959; 37: 911–917.
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[13] Beuckels A, Smolders E, Muylaert K. Nitrogen availability influences phosphorus removal in microalgae-based wastewater treatment. Water Research, 2015; 77(15): 98–106.
[14] Mohan D, Sarswat A, Ok Y S, Pittman C U J. Organic and inorganic contaminants removal from water with biochar, a renewable, low cost and sustainable adsorbent—A critical review. Bioresource Technology, 2014; 160: 191–202.
[15] Pancha I, Chokshi K, George B, Ghosh T, Paliwal C, Maurya R, et al. Nitrogen stress triggered biochemical and morphological changes in the microalgae Scenedesmus sp. CCNM 1077. Bioresource Technology, 2014; 156: 146–154.
[16] Takagi M, Watanabe K, Yamaberi K, Yoshida T. Limited feeding of potassium nitrate for intercellular lipid and triglyceride accumulation of Nannochloris sp. UTEX LB1999. Applied Microbiology Biotechnology, 2000; 54: 112–117.
[17] Meher L, VidyaSagar D, Naik S. Technical aspects of biodiesel production by transesterification-a review. Renewable Sustainable Energy Review, 2006; 10: 248–268.
[18] Singh B, Guldhe A, Rawat I, Bux F. Towards a sustainable approach for development of biodiesel from plant and microalgae. Renewable Sustainable Energy Review, 2014; 29: 216–245.
[19] Zheng Y P, Huang Y, Liao Q, Zhu X, Fu Q, Xia A. Effect of wettability on the growth of Scenedesmus obliquus biofilm attached on glass surface coated with polytetrafluoroethylene emulsion. Int. J. Hydrogen Energ., 2016; 41(46): 21728–21735.
[2] Chisti Y. Biodiesel from microalgae. Biotechnology Advances, 2007; 25(3): 294–306.
[3] Sialve B, Bernet N, Bernard O. Anaerobic digestion of microalgae as a necessary step to make microalgal biodiesel sustainable. Biotechnology Advances, 2009; 27(4): 409–416.
[4] Abinandan S, Shanthakumar S. Challenges and opportunities in application of microalgae (Chlorophyta) for wastewater treatment: A review. Renewable Sustainable Energy Reviews, 2015; 52: 123–132.
[5] Pancha I, Chokshi K, Maurya R, Trivedi K, Patidar S K, Ghosh A, et al. Salinity induced oxidative stress enhanced biofuel production potential of microalgae Scenedesmus sp. CCNM 1077. Bioresource Technology, 2015; 189: 341–348.
[6] Jiang L, Luo S, Fan X, Yang Z, Guo R. Biomass and lipid production of marine microalgae using municipal wastewater and high concentration of CO2. Applied Energy, 2011; 88: 3336–3341.
[7] Ji F, Liu Y, Hao R, Li G, Zhou Y, Dong R. Biomass production and nutrients removal by a new microalgae strain Desmodesmus sp. in anaerobic digestion wastewater. Bioresource Technology, 2014; 161: 200–207.
[8] Sriram S, Seenivasan R. Biophotonic perception on Desmodesmus sp. VIT growth, lipid and carbohydrate content. Bioresource Technology, 2015; 198: 626–633.
[9] Ebrahimian A, Kariminia HR, Vosoughi M. Lipid production in mixotrophic cultivation of Chlorella vulgaris in a mixture of primary and secondary municipal wastewater. Renewable Energy, 2014; 71: 502–508.
[10] Chang H X, Fu Q, Huang Y, Xia A, Liao Q, Zhu X, et al. An annular photobioreactor with ion-exchange-membrane for non-touch microalgae cultivation with wastewater. Bioresource Technology, 2016; 219: 668–676.
[11] Bligh E G, Dyer W J. A rapid method of total lipid extraction and purification. Canadian Journal Biochemistry Physiology, 1959; 37: 911–917.
[12] Miao X, Wu Q. Biodiesel production from heterotrophic microalgae oil. Bioresource Technology, 2006; 97(6): 841–846.
[13] Beuckels A, Smolders E, Muylaert K. Nitrogen availability influences phosphorus removal in microalgae-based wastewater treatment. Water Research, 2015; 77(15): 98–106.
[14] Mohan D, Sarswat A, Ok Y S, Pittman C U J. Organic and inorganic contaminants removal from water with biochar, a renewable, low cost and sustainable adsorbent—A critical review. Bioresource Technology, 2014; 160: 191–202.
[15] Pancha I, Chokshi K, George B, Ghosh T, Paliwal C, Maurya R, et al. Nitrogen stress triggered biochemical and morphological changes in the microalgae Scenedesmus sp. CCNM 1077. Bioresource Technology, 2014; 156: 146–154.
[16] Takagi M, Watanabe K, Yamaberi K, Yoshida T. Limited feeding of potassium nitrate for intercellular lipid and triglyceride accumulation of Nannochloris sp. UTEX LB1999. Applied Microbiology Biotechnology, 2000; 54: 112–117.
[17] Meher L, VidyaSagar D, Naik S. Technical aspects of biodiesel production by transesterification-a review. Renewable Sustainable Energy Review, 2006; 10: 248–268.
[18] Singh B, Guldhe A, Rawat I, Bux F. Towards a sustainable approach for development of biodiesel from plant and microalgae. Renewable Sustainable Energy Review, 2014; 29: 216–245.
[19] Zheng Y P, Huang Y, Liao Q, Zhu X, Fu Q, Xia A. Effect of wettability on the growth of Scenedesmus obliquus biofilm attached on glass surface coated with polytetrafluoroethylene emulsion. Int. J. Hydrogen Energ., 2016; 41(46): 21728–21735.
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Published
2017-01-23
How to Cite
Haixing, C., Qian, F., Yun, H., Ao, X., Qiang, L., & Xun, Z. (2017). Improvement of microalgae lipid productivity and quality in an ion-exchange-membrane photobioreactor using real municipal wastewater. International Journal of Agricultural and Biological Engineering, 10(1), 97–106. Retrieved from https://ijabe.migration.pkpps03.publicknowledgeproject.org/index.php/ijabe/article/view/2706
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Biosystems, Biological and Ecological Engineering
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