Effects of chlorine dioxide on morphology and ultrastructure of Fusarium sulphureum and its virulence to potato tubers
Keywords:
potato, dry rot, chlorine dioxide, Fusarium sulphureum, virulence, morphology and ultrastructureAbstract
The inhibitory effect and its virulence of chlorine dioxide (ClO2) against dry rot of potato were investigated. Potatoes were treated by ClO2, then observed for indoor bioassay, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to observe the morphology and ultrastructure of hyphae, and evaluated the control efficiency of ClO2 on potato tuber (LK99) dry rot by F. sulphureum pre-treatment. The results showed that the pathogen of potato dry rot was sensitive to ClO2, the virulence of regression of y = 5.05 + 7.308x, EC50 and EC90 were 0.3490 and 0.6261 respectively, the treatment of ClO2 could significantly inhibit the spore germination and mycelium growth of F. sulphureum, which was in a concentration-dependent manner, SEM and TEM observed that the morphology and ultrastructure of F. sulphureum hyphae were regularly damaged by ClO2, in vivo experiment further indicated that ClO2 could effectively control the dry rot of potato tubers with F. sulphureum, and ClO2 at the concentration of 0.75 ug/mL could significantly reduce the incidence of potato tuber dry rot and lesion expansion rate. The study showed that ClO2 could greatly against the pathogen of F. sulphureum, which could provide a scientific theoretical basis for the safe and efficient application of ClO2 in the prevention and control of potato diseases after harvest. Keywords: potato, dry rot, chlorine dioxide, Fusarium sulphureum, virulence, morphology and ultrastructure DOI: 10.25165/j.ijabe.20171005.2403 Citation: Li M, Tian S L, Shen J, Wang X Z, Cheng J X, Li S Q, et al. Effects of chlorine dioxide on morphology and ultrastructure of Fusarium sulphureum and its virulence to potato tubers. Int J Agric & Biol Eng, 2017; 10(5): 242–250.References
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[28] Kang Z S, Huang L L, Krieg U, Mauler-Machnik A, Buchnauer H. Effects of tebuconazole on morphology, structure, cell wall components and trichothecene production of Fusarium culmorum in vitro. Pest Management Science, 2001; 57(6): 491–500.
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[2] Yang Z M, Bi Y, Li Y C, Kou Z H, Bao G H, Liu C K, et al. Changes of cell wall degrading enzymes in potato tuber tissue slices infected by Fusarium sulphureum. Scientia Agricultura Sinica, 2012; 45(1): 127–134. (in Chinese)
[3] Li Y C, Bi Y, Ge Y H, Sun X J, Wang Y. Antifungal activity of sodium silicate on Fusarium sulphureum and its effect on dry rot of potato tubers. Journal of Food Science, 2009; 74(5): 213–218.
[4] Bi Y, Li Y C, Ge Y H. Induced resistance in postharvest fruits and vegetables by chemicals and its mechanism. Stewart Postharvest Review, 2007; 3(6): 1–7.
[5] Sun X J, Bi Y, Li Y C, Han R F, Ge Y H. Postharvest chitosan treatment induces resistance in potato against Fusarium sulphureum. Agricultural Sciences in China, 2008; 7: 615–621.
[6] Hu L G, Li Y C, Bi Y, Li J P, Bao G H, Liu J J, et al. Effects of nitric oxide on growth of Fusarium sulphureum and its virulence to potato tubers. European Food Research and Technology, 2014; 238(6): 1007–1014.
[7] Zhang Q C, Li Y C, Bi Y, Sun X J, Wang H J. The inhibiting effect of postharvest citric acid treatment on dry rot of wounded-inoculated potato and the activity of defense enzymes. Journal of Gansu Agricultural University, 2009; 3: 146–150. (in Chinese)
[8] Mur L A, Mandon J, Persijn S, Cristescu S M, Moshkov I E, Novikova G V, et al. Nitric oxide in plants: an assessment of the current state of knowledge. AoB Plants, 2013; 5: pls052.
[9] Tossi V, Amenta M, Lamattina L, Cassia R. Nitric oxide enhances plant ultraviolet-B protection up-regulating gene expression of the phenylpropanoid biosynthetic pathway. Plant, Cell & Environment, 2011; 34(6): 909–921.
[10] Schairer D O, Chouake J S, Nosanchuk J D, Friedman A J. The potential of nitric oxide releasing therapies as antimicrobial agents. Virulence, 2012; 3(3): 271–279.
[11] Lai T F, Li B Q, Qin G Z, Tian S P. Oxidative damage involves in the inhibitory effect of nitric oxide on spore germination of Penicillium expansum. Current Microbiology, 2011; 62(1): 229–234.
[12] Chen S F, Guo P Y, Ruan B. Effects of stable chlorine
dioxide on chlorophyll a and antioxiant enzymes of Microcystis flos-aquae. Journal of Central South University (Science and Technology), 2016; 47(2): 414–419. (in Chinese)
[13] Gómez-López V M, Devlieghere F, Ragaert P, Debevere J. Shelf-life extension of minimally processed carrots by gaseous chlorine dioxide. International Journal of Food Microbiology, 2007; 116 (2): 221–227.
[14] Li J K, Zhang P, Zhang P. Research progress in application of chlorine dioxide in fruit fresh-keeping. Science and Technology of Food Industry, 2011; 32(9): 439–442. (in Chinese)
[15] Zhang X, Wang X L, Plant Chemical Protection Experiment Iinstruction. Yangling: Northwest Agriculture and Forestry University of Science and Technology Press, 2000; pp.64–66. (in Chinese)
[16] Li Y C, Sun X J, Bi Y, Ge Y H, Wang Y. Antifungal activity of chitosan on Fusarium sulphureum in relation to dry rot of potato tuber. Agricultural Sciences in China, 2009; 8(5): 597–604.
[17] Yao H J, Tian S P. Effects of a biocontrol agent and methyl jasmonate on postharvest diseases of peach fruit and the possible mechanisms involved. Journal of Applied Microbiology, 2005; 98(4): 941–950.
[18] Benhamou N, Rey P, Picard K, Tirilly Y. Ultrastructural and cytochemical aspects of the interaction between the mycoparasite Pythium oligandrum and soilborne plant pathogens. Phytopathology, 1999; 89(6): 506–517.
[19] Huang J L, Wang L, Ren N Q, Ma F, Ju L, Liu X L, et al. Disinfection effect of chlorine dioxide on viruses, algae and animal planktons in water. Water Research, 1997; 31(3): 455–460.
[20] Tian C H, Ma X Y, Xu X F. The disinfection effect of chlorine dioxide on Pathogenes of Silkworm, Bombyx Mori L. Journal of Anhui Agricultural University, 1996; 4: 525–528. (in Chinese)
[21] Winiecka-Krusnell J, Linder E. Cysticidal effect of chlorine
dioxide on Giardia intestinalis cysts. Acta Tropica, 1998; 70(3): 369–372.
[22] Lu C Y, Deng X Q, Zhao M, Zhou X P. Examination of efficacy of chlorine dioxide solution in killing skin fungi. Journal of Chinese Disinfection, 2000; 17(4): 227–229. (in Chinese)
[23] Li J W, Xin Z T, Wang X W, Zheng J L, Chao F H. Mechanisms of inactivation of hepatitis A virus in water by chlorine dioxide. Water Research, 2004; 38(6): 1514–1519.
[24] Deng J H, Wu Q P, Que S H, Zheng W H, Liao F Y. Experimental observation on efficacy of chlorine dioxide in killing Chlorella. Chinese Journal of Disinfection, 2005; 22(3): 282–283. (in Chinese)
[25] Li Y C, Bi Y, Ge Y H, Sun X J, Wang Y. Antifungal activity of sodium silicate on Fusarium sulphureum and its effect on dry rot of potato tubers.Journal of Food Science, 2009; 74(5): 213–218.
[26] Qin G Z, Zong Y Y, Chen Q L, Hua D L, Tian S P. Inhibitory effect of boron against Botrytis cinerea on table grapes and its possible mechanisms of action. International Journal of Food Microbiology, 2010; 138(1-2): 145–150.
[27] Lai T F, Li B Q, Qin G Z, Tian S P. Oxidative damage involves in the inhibitory effect of nitric oxide on spore germination of Penicillium expansum. Current Microbiology, 2011; 62(1): 229–234.
[28] Kang Z S, Huang L L, Krieg U, Mauler-Machnik A, Buchnauer H. Effects of tebuconazole on morphology, structure, cell wall components and trichothecene production of Fusarium culmorum in vitro. Pest Management Science, 2001; 57(6): 491–500.
[29] Ait Barka E, Eullaffroy P, Clement C, Vernet G. Chitosan improves development, and protects Vitis vinifera L. against Botrytis cinerea. Plant Cell Reports, 2004; 22(8): 608–614.
[30] Karabulut O A, Ilhan K, Arslan U, Vardar C. Evaluation of the use of chlorine dioxide by fogging for decreasing postharvest decay of fig. Postharvest Biology and Technology, 2009; 52(3): 313–315.
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2017-09-30
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Mei, L., Shilong, T., Jin, S., Xizhuo, W., Jianxin, C., Shouqiang, L., … Jiachun, T. (2017). Effects of chlorine dioxide on morphology and ultrastructure of Fusarium sulphureum and its virulence to potato tubers. International Journal of Agricultural and Biological Engineering, 10(5), 242–250. Retrieved from https://ijabe.migration.pkpps03.publicknowledgeproject.org/index.php/ijabe/article/view/2403
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Agro-product and Food Processing Systems
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