Predicting wheat kernels’ protein content by near infrared hyperspectral imaging
Keywords:
wheat kernels, protein, nondestructive prediction, near infrared hyperspectral imaging, partial least squares regression, radial basis function neural networkAbstract
The objective of this study was to explore the potential of near infrared hyperspectral imaging combined with statistical regression models and neural networks for nondestructive prediction of protein content of wheat kernels. Seventy-nine samples from 11 breeds of wheat kernels were collected. The protein percentage of each sample measured by semimicro-Kjeldahl method was taken as the reference value. After comparing the prediction models of principal components regression (PCR) and partial least squares regression (PLSR) with various pretreatment methods, PLSR preprocessed by zero mean normalization (z score) function of MATLAB was found to obtain better prediction results than other regression models. Based on 10 latent variables of PLSR, the radial basis function (RBF) neural network was applied to improve the prediction, in which the coefficients of determination (R2) were greater than 0.92 for both the calibration set and validation set, while the corresponding RMSE values were 0.3496 and 0.4005, respectively. Therefore, hyperspectral imaging can provide a fast and non-destructive method for predicting the wheat kernels’ protein content. Keywords: wheat kernels, protein, nondestructive prediction, near infrared hyperspectral imaging, partial least squares regression, radial basis function neural network DOI: 10.3965/j.ijabe.20160902.1701 Citation: Yang S Q, He D J, Ning J F. Predicting wheat kernels’ protein content by near infrared hyperspectral imaging. Int J Agric & Biol Eng, 2016; 9(2): 163-170.References
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[17] Fawcett J K. The semi-micro Kjeldahl method for the determination of nitrogen. The Journal of Medical Laboratory Technology, 1954; 12(1): 1–22.
[18] Chang S K. Protein analysis. Food analysis. Springer, US, 2010. Chapter 9, pp.133–146.
[2] Serrano S, Rincón F, García-Olmo J. Cereal protein analysis via Dumas method: Standardization of a micro-method using the EuroVector Elemental Analyser. Journal of Cereal Science, 2013; 58(1): 31–36.
[3] Itzhaki R F, Gill D M. A micro-biuret method for estimating proteins. Analytical Biochemistry, 1964; 9(4): 401–410.
[4] Markwell M A K, Haas S M, Bieber L L, Tolbert N. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Analytical Biochemistry, 1978; 87(1): 206–210.
[5] Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 1976; 72(1): 248–254.
[6] Bogomolov A, Dietrich S, Boldrini B, Kessler R W. Quantitative determination of fat and total protein in milk based on visible light scatter. Food Chemistry, 2012; 134(1): 412–418.
[7] Kays S E, Barton I I, Franklin E, Windham W R. Predicting protein content by near infrared reflectance spectroscopy in diverse cereal food products. Journal of Near Infrared Spectroscopy, 2000; 8(1): 35–43.
[8] Pohl F, Senn T. A rapid and sensitive method for the evaluation of cereal grains in bioethanol production using near infrared reflectance spectroscopy. Bioresource Technology, 2011; 102(3): 2834–2841.
[9] Norgaard L, Lagerholm M, Westerhaus M. Artificial Neural Networks and Near Infrared Spectroscopy-A case study on protein content in whole wheat grain. Foss White Paper. Available from: http://www.foss.dk/campaign/-/media/ 242657904D734CE9B0652C3D885776AE.ashx. Accessed on [2014-12-30]
[10] Sun D W. Hyperspectral imaging for food quality analysis and control. Elsevier, USA, 2010.
[11] Huang H, Liu L, Ngadi M O. Recent developments in hyperspectral imaging for assessment of food quality and safety. Sensors, 2014; 14(4): 7248–7276.
[12] Wu D, Sun D W. Advanced applications of hyperspectral imaging technology for food quality and safety analysis and assessment: A review—Part I: Fundamentals. Innovative Food Science & Emerging Technologies, 2013; 19: 1–14.
[13] Barbin D F, ElMasry G, Sun D W, Allen P. Non-destructive determination of chemical composition in intact and minced pork using near-infrared hyperspectral imaging. Food Chemistry, 2013; 138(2): 1162–1171.
[14] Bhuvaneswari K, Fields P G, White N D, Sarkar A K, Singh C B, Jayas D S. Image analysis for detecting insect fragments in semolina. Journal of Stored Products Research, 2011; 47(1): 20–24.
[15] Serranti S, Cesare D, Marini F, Bonifazi G. Classification of oat and groat kernels using NIR hyperspectral imaging. Talanta, 2013; 103: 276–284.
[16] Choudhary R, Mahesh S, Paliwal J, Jayas D S. Identification of wheat classes using wavelet features from near infrared hyperspectral images of bulk samples. Biosystems Engineering, 2009; 102(2): 115–127.
[17] Fawcett J K. The semi-micro Kjeldahl method for the determination of nitrogen. The Journal of Medical Laboratory Technology, 1954; 12(1): 1–22.
[18] Chang S K. Protein analysis. Food analysis. Springer, US, 2010. Chapter 9, pp.133–146.
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Published
2016-03-31
How to Cite
Shuqin, Y., Dongjian, H., & Jifeng, N. (2016). Predicting wheat kernels’ protein content by near infrared hyperspectral imaging. International Journal of Agricultural and Biological Engineering, 9(2), 163–170. Retrieved from https://ijabe.migration.pkpps03.publicknowledgeproject.org/index.php/ijabe/article/view/1701
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Agro-product and Food Processing Systems
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